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Purified anti-mouse/rat CD29 Antibody

Pricing & Availability
Clone
HMβ1-1 (See other available formats)
Regulatory Status
RUO
Other Names
integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMbeta1-1_PURE_anti-mouse-rat-CD29_Antibody_1_FC_102804
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE.
  • HMbeta1-1_PURE_anti-mouse-rat-CD29_Antibody_1_FC_102804
    Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE.
  • HMbeta1-1_PURE_anti-mouse-rat-CD29_Antibody_2_FC_102804
    C57BL/6 mouse splenocytes stained with HMβ1-1 PE.
  • HMbeta1-1_PURE_anti-mouse-rat-CD29_Antibody_4_IHCF_010419
    Rat frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD4 (clone W3/25) Alexa Fluor® 488 (green), 10 µg/mL of CD45RC (clone OX-22) Alexa Fluor® 647 (blue), and 10 µg/mL of purified CD29 (clone HMβ1-1) overnight at 4°C, followed by 5 µg/mL of anti-Armenian hamster IgG (clone Poly4055) Alexa Fluor® 594 (red) for two hours at room temperature. The image was captured by 10X objective.
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102201 50 µg 67€
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102202 500 µg 207€
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Description

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102235 and 102236).

Application References

(PubMed link indicates BioLegend citation)
  1. Noto K, et al. 1995. Int. Immunol. 7:835.
  2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
  3. Jia W, et al. 2005. Blood 106:3854.PubMed
  4. Economopoulou M, et al. 2005. Blood 106:3831.
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
  7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
  8. Kim DT, et al. 2008. Blood 111:2929.PubMed
  9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
  10. Carlson TR, et al. 2008. Development. 135:2193. PubMed
  11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
  12. Baker CM, et al. 2012. PNAS. PubMed.
  13. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Roblek M, et al. 2022. Front Oncol. 12:777634. PubMed
  3. Mirgorodskaya E, et al. 2022. Biol Cell. 114:160. PubMed
  4. Jia W, et al. 2005. Blood. 106:3854. PubMed
  5. García-Bernal D, et al. 2022. NPJ Regen Med. 7:61. PubMed
  6. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
  7. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  8. Högnäs G, et al. 2011. Oncogene. 1.1375. PubMed
  9. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  10. Xi L, et al. 2020. Elife. 9:00. PubMed
  11. Bieniasz–Krzywiec P, et al. 2020. Cell Metabolism. 30(5):917-936. PubMed
  12. Hook JL, et al. 2018. J Clin Invest. 2018:5379085. PubMed
  13. Kim BR, et al. 2017. PLoS One. 12:e0187464. PubMed
  14. Eisenmann K, et al. 2007. J Biol Chem. 282:25152. PubMed
  15. Ong T, et al. 2020. Nat Commun. 11:5312. PubMed
  16. Freeman SA, et al. 2020. Science. 367:301. PubMed
  17. Carlson T, et al. 2008. Development. 135:2193. PubMed
  18. Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  19. Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed
  20. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
  21. Baker C, et al. 2012. Proc Natl Acad Sci U S A. 109:10474. PubMed
RRID
AB_312878 (BioLegend Cat. No. 102201)
AB_312878 (BioLegend Cat. No. 102202)

Antigen Details

Structure
Integrin family, 130 kD
Distribution

Leukocytes, endothelial cells, smooth muscle, epithelial cells

Function
Adhesion
Ligand/Receptor
Extracellular matrix
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.

Gene ID
16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 01.04.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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