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PE anti-human Siglec-9 Antibody

Pricing & Availability
Clone
K8 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid-binding Ig-like lectin-9, CD329
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
K8_PE_030411
Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
  • K8_PE_030411
    Human peripheral blood granulocytes were stained with Siglec-9 (clone K8) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
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Cat # Size Price Quantity Check Availability Save
351503 25 tests £86
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351504 100 tests £172
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Description

Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-9 fused to Fc region of human IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang J, et al. 2000. J. Biol. Chem. 275:22121.
  2. Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB)
  3. Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)
Product Citations
  1. Jobe O, et al. 2016. J Leukoc Biol. 99: 1089 - 1106. PubMed
RRID
AB_10719225 (BioLegend Cat. No. 351503)
AB_10719225 (BioLegend Cat. No. 351504)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells

Ligand/Receptor
Sialic acid
Cell Type
Monocytes, Neutrophils
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.

Gene ID
27180 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Siglec-9
Specificity Alt (DOES NOT SHOW ON TDS):
Siglec-9
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about Siglec-9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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