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Alexa Fluor® 647 anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
PAC-1 (See other available formats)
Regulatory Status
RUO
Other Names
gpIIb/IIIa complex, integrin αIIbβ3
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
PAC-1_A647_CD41slashCD61_Antibody_FC_1_102315
ADP activated enriched human platelets (top) or resting platelets (bottom) were co-stained with CD62P PE and anti-human CD41/CD61 (glycoprotein IIb/IIIa) complex (clone PAC-1) Alexa Fluor® 647.
  • PAC-1_A647_CD41slashCD61_Antibody_FC_1_102315
    ADP activated enriched human platelets (top) or resting platelets (bottom) were co-stained with CD62P PE and anti-human CD41/CD61 (glycoprotein IIb/IIIa) complex (clone PAC-1) Alexa Fluor® 647.
  • PAC-1_A647_CD41slashCD61_Antibody_FC_2_102315
Compare all formats See Alexa Fluor® 647 spectral data
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362805 25 tests £113
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362806 100 tests £254
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Description

The gpIIb/IIIa complex, also known as integrin αIIbβ3, is expressed by megakaryocytes and platelets. It undergoes conformational changes upon activation by substances, such as ADP, thrombin, and TXA2, which subsequently reveals a ligand binding site that is specific for fibrinogen, von Willebrand factor (vWF), fibronectin, and vitronectin. This conformational change is crucial for platelet-platelet adhesion. Ligand bound gpIIb/IIIa complex promotes platelet aggregation. This complex plays an important role in hemostasis and is a target for developing therapeutic antibodies in immune thrombocytopenia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation1,2, Western blotting2 and in vitro blocking of platelet aggregation2.

 

The PAC-1 antibody reacts with activation-induced conformational epitope of the integrin aIIbß3. PAC-1 binding to fibrinogen is pH and Ca2+ dependent, and will not bind to platelets that have been fixed.  We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher.  Sodium citrate may be used as an anticoagulant for blood collection3.

Application References

(PubMed link indicates BioLegend citation)
  1. Shattil SJ, et al. 1987. Blood. 70:307. (IP)
  2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107. (Block, IP, WB)
  3. Shattil SJ, et al. 1986. Blood. 68: 1224-31.
Product Citations
  1. Van Bael J, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Ma X, et al. 2023. Research (Wash D C). 6:0124. PubMed
  3. Yasseen BA, et al. 2022. Front Immunol. 13:1022401. PubMed
  4. Chauhan A, et al. 2020. Nat Commun. 11:1939. PubMed
  5. Nicolai L, et al. 2020. Circulation. 142:1176. PubMed
  6. Gillespie S, et al. 2018. Br J Pharmacol. 175:3333. PubMed
  7. Bahmani B, et al. 2021. Nat Commun. 12:1999. PubMed
  8. Chen Z, et al. 2019. Nat Commun. 10:2051. PubMed
RRID
AB_2566475 (BioLegend Cat. No. 362805)
AB_2566475 (BioLegend Cat. No. 362806)

Antigen Details

Structure
α/β transmembrane heterodimer of the ubiquitous integrin superfamily
Distribution

Resting platelets; activated platelets express a conformationally modified complex that can bind fibrin

Function
Fibrinogen binding, platelet aggregation
Ligand/Receptor
Fibrinogen
Cell Type
Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hynes RO. 1992. Cell 69:11.
2. Shattil SJ, et al. 1985. J. Biol. Chem. 260:11107.
3. Ginsberg, et al. 1990. Blood 76:2017.
4. O’Toole TE, et al. 1991. Science 254:845.
5. Peterson JA, et al. 1998. Blood 92: 2053.

Gene ID
3674 View all products for this Gene ID 3690 View all products for this Gene ID
UniProt
View information about CD41 or CD61 on UniProt.org
Go To Top Version: 1    Revision Date: 10/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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