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Alexa Fluor® 647 anti-LAT Phospho (Tyr171) Antibody

Pricing & Availability
Clone
A20005D (See other available formats)
Regulatory Status
RUO
Other Names
Linker For Activation Of T Cells; P36-38
Isotype
Mouse IgG2b, κ
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Product Citations
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A20005D_A647_Phospho_Antibody_1_110222
Jurkat cells untreated (negative control, open histogram) or treated with 5 mM of H2O2 for 3 minutes (positive control, solid histogram) were fixed and permeabilized using True Phos Perm Buffer (Cat No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-LAT Phospho (Tyr 171) (clone A20005D), or Alexa Fluor® 647 Mouse IgG2b, κ isotype control (open histogram, dashed line)(representative histogram for untreated and H2O2 treated Jurkat cells) (Cat No. 400330).
  • A20005D_A647_Phospho_Antibody_1_110222
    Jurkat cells untreated (negative control, open histogram) or treated with 5 mM of H2O2 for 3 minutes (positive control, solid histogram) were fixed and permeabilized using True Phos Perm Buffer (Cat No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-LAT Phospho (Tyr 171) (clone A20005D), or Alexa Fluor® 647 Mouse IgG2b, κ isotype control (open histogram, dashed line)(representative histogram for untreated and H2O2 treated Jurkat cells) (Cat No. 400330).
  • A20005D_A647_Phospho_Antibody_2_110222
    Peripherical blood mononuclear cells (PBMC) untreated (left), or treated with 5mM of H2O2 for 3 minutes (right) were fixed and permeabilizated using True Phos Perm Buffer (Cat No. 425401), and then intracellular stained with Alexa Fluor® 647 anti-LAT Phospho (Tyr 171) (clone A20005D), and Alexa Fluor® 488 anti-CD3 (Cat No. 317310).
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946603 25 tests £136
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946604 100 tests £321
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Description

LAT, or Linker for Activation of T cells, is a transmembrane protein expressed mainly in T cells and a limited number of other immune cells such as natural killer cells, mast cells, and immature B cells. As an adaptor protein, phosphorylated LAT facilitates the recruitment of other signaling proteins and functions as a nucleating site for multiprotein signaling complexes. These signaling complexes can propagate TCR, or T Cell antigen Receptors, and activate downstream effectors, which trigger T cell proliferation or cytokine expression.

LAT (Tyr171) is one of the nine tyrosines in LAT conserved between human, mice, and rat. LAT (Tyr171) is necessary for the activation of PI 3-kinase, a protein that regulates the recruitment of other proteins that facilitate in T-cell receptor signal transduction such as PLC-gamma1 and Sos1. When LAT (Tyr171) and (Tyr191) is phosphorylated by ZAP-70, Gads can bind directly to LAT (Tyr171) and (Tyr191) and mediate the recruitment of SLP-76. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above.

This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation/permeabilization method produced the best staining.

This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC

RRID
AB_2927971 (BioLegend Cat. No. 946603)
AB_2927971 (BioLegend Cat. No. 946604)

Antigen Details

Structure
LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.
Distribution

Plasma membrane/T Cells

Function
Plasma membrane/T Cells
Cell Type
Mast cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References
  1. Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512
  2. Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036
  3. Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95
  4. Paz et. al., 2001, Biochem J., 356, 461-71
  5. Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61
Gene ID
27040 View all products for this Gene ID
UniProt
View information about LAT Phospho Tyr171 on UniProt.org
Go To Top Version: 1    Revision Date: 10/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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