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PE anti-PLCγ1 Phospho (Tyr783) Antibody

Pricing & Availability
Clone
A17025A (See other available formats)
Regulatory Status
RUO
Other Names
PLC1, NCKAP3, PLC-II, PLC148
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17025A_PE_Phospho_Tyr783_Antibody_dotplot_011519_updated.png
Human peripheral blood lymphocytes were treated with (left), or without (right) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), then surfaced stained with CD20 FITC and intracellularly stained with anti-PLCγ1 Phospho (Tyr783) (clone A17025A) PE.
  • A17025A_PE_Phospho_Tyr783_Antibody_dotplot_011519_updated.png
    Human peripheral blood lymphocytes were treated with (left), or without (right) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), then surfaced stained with CD20 FITC and intracellularly stained with anti-PLCγ1 Phospho (Tyr783) (clone A17025A) PE.
  • A17025A_PE_Phospho_Tyr783_Antibody_histogram_011519.png
    Human T lymphoblastic leukemia cell line, Jurkat, was stimulated with (filled histogram) or without (open histogram) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), and intracellularly stained with anti-PLCγ1 Phospho (Tyr783) (clone A17025A) PE.
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612403 25 tests £105
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612404 100 tests £247
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Description

Phospholipase C (PLC) enzymes cleave the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into second messenger molecules inositol 1,4,5-triposphate (IP3) and diacylglycerol (DAG) in response to extracellular stimuli. IP3 causes the release of calcium from the endoplasmic reticulum to begin an intracellular signaling cascade, while DAG activates Protein Kinase C.

There are four families of PLCs. PLC gamma (PLCγ) uniquely contains SRC domains SH2 and SH3. Receptor tyrosine kinases interact with these SRC domains to enzymatically activate PLCγ1 via phosphorylation at tyrosine 783. PLCγ1 is essential for cell proliferation, differentiation, and motility.  Overexpression or activation of PLCγ1 has been implicated in breast and prostate cancers as well as cancer cell invasion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human PLCγ1 phosphorylated at Tyr783 emulsified in CFA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2801119 (BioLegend Cat. No. 612403)
AB_2801119 (BioLegend Cat. No. 612404)

Antigen Details

Structure
1290 and 1291 amino acid isoforms with predicted molecular weights of 148.5 and 148.7 kD, respectively
Function
T cell receptor signaling
Cell Type
T cells
Biology Area
Cell Proliferation and Viability, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Brady-Kalnay, et al. 2011. J Cell Biochem. 112:39.
  2. Schlessinger, et al. 2009. Cell. 138:514.
  3. Harden, et al. 2012. Subcell Biochem. 58:61.
  4. Wang Z, et al. 1998. Mol Cell Biol. 18:590.
  5. Rhee, et al. 1991. Cell. 65:435.
Gene ID
5335 View all products for this Gene ID
UniProt
View information about PLCgamma1 Phospho Tyr783 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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