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Alexa Fluor® 647 anti-mouse Blimp-1 Antibody

Pricing & Availability
Clone
5E7 (See other available formats)
Regulatory Status
RUO
Other Names
PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5E7_AF647_Blimp-1_Antibody_FC_1_042315
Balb/c spleen cells were cultured four days in presence of LPS, then were stained with CD45R/B220 PE and IgD Brilliant Violet 421™, followed by fixation and permeabilization with True-Nuclear™ Transcription Factor Buffer Set, and staining with Blimp-1 (clone 5E7) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • 5E7_AF647_Blimp-1_Antibody_FC_1_042315
    Balb/c spleen cells were cultured four days in presence of LPS, then were stained with CD45R/B220 PE and IgD Brilliant Violet 421™, followed by fixation and permeabilization with True-Nuclear™ Transcription Factor Buffer Set, and staining with Blimp-1 (clone 5E7) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • 5E7_AF647_Blimp-1_Antibody_FC_2_042315
Compare all formats See Alexa Fluor® 647 spectral data
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150003 25 µg £113
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150004 100 µg £257
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Description

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Chen HY, et al. 2022. Elife. 11:. PubMed
  2. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  3. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  4. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  5. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  6. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  7. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  8. Roy K, et al. 2019. Immunity. 50:616. PubMed
  9. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  10. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  11. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  12. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  13. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
RRID
AB_2565617 (BioLegend Cat. No. 150003)
AB_2565617 (BioLegend Cat. No. 150004)

Antigen Details

Structure
Five Kruppel-type zinc finger domains, 98 kD.
Distribution

Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.

Function
Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type
Plasma cells, T cells, NK cells, B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Nakaki F, et al. 2013. Nature 501:222.
2. Crotty S, et al. 2010. Nat. Immunol. 11:114.
3. Zhao WL, et al. 2008. Blood 111:3867.
4. Climmino L, et al. 2008. J. Immunol. 181:2338.
5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.

Gene ID
12142 View all products for this Gene ID
UniProt
View information about Blimp-1 on UniProt.org
Go To Top Version: 1    Revision Date: 05/01/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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