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APC anti-mouse Blimp-1 Antibody

Pricing & Availability
Clone
5E7 (See other available formats)
Regulatory Status
RUO
Other Names
PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5E7_APC_Blimp-1_Antibody_032718
Balb/c splenocytes were cultured 3 days in the presence of LPS, then stained with CD45R/B220 FITC, followed by fixation and permeabilization using True-Nuclear™ fixation and permeabilization buffer set. Cells were then stained with Blimp-1 (clone 5E7) APC (left) or rat IgG2a, κ Isotype control APC (right).
  • 5E7_APC_Blimp-1_Antibody_032718
    Balb/c splenocytes were cultured 3 days in the presence of LPS, then stained with CD45R/B220 FITC, followed by fixation and permeabilization using True-Nuclear™ fixation and permeabilization buffer set. Cells were then stained with Blimp-1 (clone 5E7) APC (left) or rat IgG2a, κ Isotype control APC (right).
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150007 25 µg £102
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150008 100 µg £240
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Description

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Dean JW 2023. Cell Reports. 42(1):111963. PubMed
  2. Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed
  3. Pioli PD, et al. 2020. Immunity. 51(2):351-366. PubMed
  4. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  5. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
RRID
AB_2728186 (BioLegend Cat. No. 150007)
AB_2728186 (BioLegend Cat. No. 150008)

Antigen Details

Structure
Five Kruppel-type zinc finger domains, 98 kD.
Distribution

Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.

Function
Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type
Plasma cells, T cells, NK cells, B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Nakaki F, et al. 2013. Nature 501:222.
2. Crotty S, et al. 2010. Nat. Immunol. 11:114.
3. Zhao WL, et al. 2008. Blood 111:3867.
4. Climmino L, et al. 2008. J. Immunol. 181:2338.
5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.

Gene ID
12142 View all products for this Gene ID
UniProt
View information about Blimp-1 on UniProt.org
Go To Top Version: 1    Revision Date: 03/28/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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