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PE anti-mouse Blimp-1 Antibody

Pricing & Availability
Clone
5E7 (See other available formats)
Regulatory Status
RUO
Other Names
PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5E7_PE_Blimp-1_Antibody_FC_021519_updated.png
C57BL/6 splenocytes were cultured for 4 days in the presence of LPS, stained with CD45R/B220 APC, fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer, then stained with Blimp-1 (clone 5E7) PE (left) or Rat IgG2a, κ PE Isotype control (right)
  • 5E7_PE_Blimp-1_Antibody_FC_021519_updated.png
    C57BL/6 splenocytes were cultured for 4 days in the presence of LPS, stained with CD45R/B220 APC, fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer, then stained with Blimp-1 (clone 5E7) PE (left) or Rat IgG2a, κ PE Isotype control (right)
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150005 25 µg £97
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150006 100 µg £238
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Description

Blimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Amino acids 255-395 from mouse Blimp-1 fused with GST.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Product Citations
  1. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  2. Chen HY, et al. 2022. Elife. 11:. PubMed
  3. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
RRID
AB_2565992 (BioLegend Cat. No. 150005)
AB_2565992 (BioLegend Cat. No. 150006)

Antigen Details

Structure
Five Kruppel-type zinc finger domains, 98 kD.
Distribution

Plasma cells, CD4+ and CD8+ effector/memory T cells, and natural killer cells.

Function
Terminal differentiation of B cells, effector/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.
Cell Type
Plasma cells, T cells, NK cells, B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Antigen References

1. Nakaki F, et al. 2013. Nature 501:222.
2. Crotty S, et al. 2010. Nat. Immunol. 11:114.
3. Zhao WL, et al. 2008. Blood 111:3867.
4. Climmino L, et al. 2008. J. Immunol. 181:2338.
5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.

Gene ID
12142 View all products for this Gene ID
UniProt
View information about Blimp-1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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