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Alexa Fluor® 700 anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
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Product Citations
publications
16A8_AF700_Ki-67_Antibody_FC_091914
Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Alexa Fluor® 700 (filled histogram). Unstained cells are represented by the open histogram.
  • 16A8_AF700_Ki-67_Antibody_FC_091914
    Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Alexa Fluor® 700 (filled histogram). Unstained cells are represented by the open histogram.
Compare all formats See Alexa Fluor® 700 spectral data
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652419 25 µg £101
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652420 100 µg £262
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
  2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  2. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  3. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  4. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  5. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  6. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  7. Hill DL, et al. 2021. Elife. 10:. PubMed
  8. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  9. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  10. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  11. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  12. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  13. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  14. Xia L, et al. 2021. Cell Chemical Biology. 28(5):610-624.e5. PubMed
  15. Song W, et al. 2022. Immunity. 55:290. PubMed
  16. Jacobs L, et al. 2022. Cancer Gene Ther. 29:984. PubMed
RRID
AB_2564284 (BioLegend Cat. No. 652419)
AB_2564284 (BioLegend Cat. No. 652420)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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