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APC anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
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Product Citations
publications
16A8_APC_Ki67_Antibody_ICFC_032613
Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • 16A8_APC_Ki67_Antibody_ICFC_032613
    Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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652405 25 µg £102
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652406 100 µg £256
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
  2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Efraim Y, et al. 2022. Cell Rep. 40:111307. PubMed
  2. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  3. Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed
  4. Bongiorno EK, et al. 2017. J Immunol. 198:4513. PubMed
  5. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  6. Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed
  7. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  8. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  9. Robinson BS, et al. 2020. Am J Pathol. 190:1657. PubMed
  10. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  11. Hsu J, et al. 2020. Proceedings of the National Academy of Sciences. 117(38):23626-23635. PubMed
  12. Wang W, et al. 2020. Cell Rep. 107936:32. PubMed
  13. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  14. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  15. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  16. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  17. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  18. Supper E, et al. 2021. Nat Commun. 12:2482. PubMed
  19. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  20. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  21. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  22. La T, et al. 2018. Cancer Res. 78:6666. PubMed
  23. Emmerson E, et al. 2018. EMBO Mol Med. 10.15252/emmm.201708051. PubMed
  24. Bainor AJ et al. 2018. Cell reports. 25(10):2797-2807 . PubMed
  25. Wesely J, et al. 2017. Stem Cells Int. 2017:5762301. PubMed
  26. Hewitt KJ et al. 2017. Developmental cell. 42(3):213-225 . PubMed
  27. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  28. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  29. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  30. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  31. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  32. Hamaidi I, et al. 2020. Cell Metabolism. 32(3):420-436.e12. PubMed
  33. Li J, et al. 2021. Transl Oncol. 14:101116. PubMed
  34. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  35. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  36. Järås M, et al. 2014. J Exp Med. 211:605. PubMed
  37. Jatho A, et al. 2022. Cells. 11:. PubMed
  38. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  39. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  40. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  41. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  42. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_2561929 (BioLegend Cat. No. 652405)
AB_2561929 (BioLegend Cat. No. 652406)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org
Go To Top Version: 4    Revision Date: 01/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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