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Anti-CD31 (PECAM-1) Antibody (Previously Covance catalog# MMS-484R)

Pricing & Availability
Clone
P2B1 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet endothelial cell adhesion molecule, PECA1, GPIIA', endoCAM, CD31 antigen, CD31/EndoCAM, adhesion molecule, PECAM-1, CD31/EndoCAM
Previously
Covance Catalog# MMS-484R
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P2B1_PECAM_Ascites_IHC_031615
Staining of PECAM using Clone P2B1 on frozen human kidney.
  • P2B1_PECAM_Ascites_IHC_031615
    Staining of PECAM using Clone P2B1 on frozen human kidney.
  • P2B1_PECAM_Ascites_IHC_2_031615
    Immunofluorescence of HUVEC cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) CD31 antibody (Clone P2B1). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/15, and (B-D) is 1/30. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml and (D) is 0.5 µg/ml.
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910003 100 µL £174
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Description

PECAM (platelet endothelial cell adhesion molecule) is expressed on activated T-cells and endothelial cells and is also known as CD31. The protein is involved in transmigration of leukocytes across endothelium during inflammation. P2B1 activates the binding of human T-lymphocytes to endothelial cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P2B1 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs).
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IHC-F - Quality tested
ICC, WB, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution range of 1:500 - 1:1000 is suggested. For immunocytochemistry, a range of 1:250 - 1:500 is recommended. For Western blotting, the suggested use of this reagent is 1:1000 dilution. For immunoprecipitation, the suggested use of this reagent is 1:1000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry (ICC), immunoprecipitation (IP) and immunohistochemistry (IHC).

*Predicted MW = 83 kD
Positive Control: human kidney

Application References

(PubMed link indicates BioLegend citation)
  1. Brooks PC, et al. 1995. J Clin Invest. 96:1815. (IF)
Product Citations
  1. Taha A, et al. 2020. J Cell Physiol. 7094:235. PubMed
  2. Zhai X, et al. 2018. Oncol Lett. 15:1893. PubMed
RRID
AB_2565394 (BioLegend Cat. No. 910003)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroinflammation, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 3    Revision Date: 10/19/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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