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Anti-E. coli RNA Sigma S Antibody

Pricing & Availability
Clone
1RS1 (See other available formats)
Regulatory Status
RUO
Other Names
Sigma 38, rpoS, appR, katF, nur, otsX, sigS, RNA Polymerase
Previously
NeoClone Catalog# W0009
Isotype
Mouse IgG1
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Product Citations
publications
1RS1_PURE_Ecoli_RNA_SigmaS_Antibody_WB_060116
Western blot analysis of 50 ng of recombinant E. coli RNA Sigma S (lane 1), 15 µg E. coli BL21 (lane 2), 15 µg E. coli DH5α (lane 3), and 15 µg E. coli Rosetta (lane 4) using anti-E. coli RNA Sigma S antibody (clone 1RS1) followed by HRP goat anti-mouse IgG secondary antibody.
  • 1RS1_PURE_Ecoli_RNA_SigmaS_Antibody_WB_060116
    Western blot analysis of 50 ng of recombinant E. coli RNA Sigma S (lane 1), 15 µg E. coli BL21 (lane 2), 15 µg E. coli DH5α (lane 3), and 15 µg E. coli Rosetta (lane 4) using anti-E. coli RNA Sigma S antibody (clone 1RS1) followed by HRP goat anti-mouse IgG secondary antibody.
Cat # Size Price Quantity Check Availability Save
663703 100 µL £221
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Description

Sigma transcription factor controlling the regulation of genes is required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon, and other genes by modifying the affinity of RNA polymerase for the promoters.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Preparation
Dried ascites
Storage & Handling
Store lyophilized antibody at -20°C.
Application

WB - Quality tested
ELISA - Verified
FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# W0009 from Neoclone.

Clone 1RS1 does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthobacter, or Borrelia. It recognizes epitope 33-256.

Additional reported applications (for the relevant formats) include: functional assay.

Additional Product Notes

Non-specific band may be observed at 60 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Nguyen LH, et al. 1993. Biochemistry 32:11112.
Product Citations
  1. Bray AS, et al. 2022. MBio. 13:e0359521. PubMed
  2. Kurylo CM, et al. 2018. Cell Rep. 25:236. PubMed
  3. Xu L et al. 2017. Structure (London, England : 1993). 25(12):1795-1808 . PubMed
RRID
AB_2564522 (BioLegend Cat. No. 663703)

Antigen Details

Structure
Sigma S (σS) is a 38 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947210 View all products for this Gene ID
UniProt
View information about RNA Sigma S on UniProt.org
Go To Top Version: 4    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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