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APC anti-human CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
DL-101 (See other available formats)
Regulatory Status
RUO
Workshop
V B045
Other Names
Syndecan-1, B-B4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DL-101_APC_062911
Human myeloma cell line U266 was stained with CD138 (clone DL-101) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • DL-101_APC_062911
    Human myeloma cell line U266 was stained with CD138 (clone DL-101) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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352307 25 tests £82
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352308 100 tests £200
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human SDC1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.2

Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC)
  2. Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. PubMed
Product Citations
  1. Yao W, et al. 2019. Nature. 568:410. PubMed
  2. Meng L, et al. 2021. Front Oncol. 11:691705. PubMed
  3. Stefka A, et al. 2016. Blood Cancer J. 6:e460. PubMed
  4. Masuda T, et al. 2022. iScience. 25:104781. PubMed
  5. Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed
  6. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  7. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  8. Drannik A, et al. 2012. J Virol. 86:4599. PubMed
  9. Wellemans V, et al. 2021. iScience. 24:103433. PubMed
  10. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  11. Aalam F, et al. 2020. PLoS Pathog. 16:e1008968. PubMed
RRID
AB_10896946 (BioLegend Cat. No. 352307)
AB_10896946 (BioLegend Cat. No. 352308)

Antigen Details

Structure
100-200 kD type I integral transmembrane glycoprotein
Distribution

Plasma cells, pre-B cells, epithelial cells, endothelial cells

Function
Adhesion, control cell morphology, regulate cell growth
Ligand/Receptor
FGFb, collagen, fibronectin
Cell Type
B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.

Gene ID
6382 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org
Go To Top Version: 2    Revision Date: 06/09/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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