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APC/Cyanine7 anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
Ave. Rating
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Product Citations
publications
PC61_APCCy7_110308.jpg
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 APC/Cyanine7
  • PC61_APCCy7_110308.jpg
    Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
102025 25 µg £80
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102026 100 µg £200
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  3. Ryoden Y, et al. 2022. Proc Natl Acad Sci U S A. 119: . PubMed
  4. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  5. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  6. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  7. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  8. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  9. Di Luccia B, et al. 2020. Cell Host & Microbe. 27(6):899-908. PubMed
  10. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  11. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  12. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  13. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  14. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  15. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  16. Duan H, et al. 2021. J Clin Invest. 131:. PubMed
  17. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  18. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  19. Olguín JE, et al. 2018. J Cancer. 9:239. PubMed
  20. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  21. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  22. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  23. Long C, et al. 2016. Toxicol Sci. 152: 85 - 98. PubMed
  24. Chan S, et al. 2018. PLoS One. 13:e0207619. PubMed
  25. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  26. Li C, et al. 2018. Cell. 174:285. PubMed
  27. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  28. Eremenko E, et al. 2019. EBioMedicine. 43:424. PubMed
  29. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  30. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  31. Morawski P, et al. 2013. J Biol Chem. 288:24494. PubMed
  32. Randall K, et al. 2011. J Exp Med. 208:2305. PubMed
  33. Rio R, et al. 2011. J Immunol. 186:382. PubMed
  34. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  35. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
RRID
AB_830744 (BioLegend Cat. No. 102025)
AB_830744 (BioLegend Cat. No. 102026)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD25 PC61 FC
Biotin anti-mouse CD25 PC61 FC
FITC anti-mouse CD25 PC61 FC
PE anti-mouse CD25 PC61 FC
PE/Cyanine5 anti-mouse CD25 PC61 FC
Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
PE/Cyanine7 anti-mouse CD25 PC61 FC
Alexa Fluor® 488 anti-mouse CD25 PC61 FC
Alexa Fluor® 647 anti-mouse CD25 PC61 FC
Pacific Blue™ anti-mouse CD25 PC61 FC
Alexa Fluor® 700 anti-mouse CD25 PC61 FC
APC/Cyanine7 anti-mouse CD25 PC61 FC
PerCP/Cyanine5.5 anti-mouse CD25 PC61 FC
PerCP anti-mouse CD25 PC61 FC
Brilliant Violet 421™ anti-mouse CD25 PC61 FC
Brilliant Violet 605™ anti-mouse CD25 PC61 FC
Brilliant Violet 650™ anti-mouse CD25 PC61 FC
Ultra-LEAF™ Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Brilliant Violet 510™ anti-mouse CD25 PC61 FC
PE/Dazzle™ 594 anti-mouse CD25 PC61 FC
Brilliant Violet 711™ anti-mouse CD25 PC61 FC
Brilliant Violet 785™ anti-mouse CD25 PC61 FC
Alexa Fluor® 594 anti-mouse CD25 PC61 IHC-F
APC/Fire™ 750 anti-mouse CD25 PC61 FC
TotalSeq™-A0097 anti-mouse CD25 PC61 PG
KIRAVIA Blue 520™ anti-mouse CD25 PC61 FC
TotalSeq™-B0097 anti-mouse CD25 PC61 PG
TotalSeq™-C0097 anti-mouse CD25 PC61 PG
Spark NIR™ 685 anti-mouse CD25 Antibody PC61 FC
PE/Fire™ 640 anti-mouse CD25 PC61 FC
Spark YG™ 581 anti-mouse CD25 PC61 FC
APC/Fire™ 810 anti-mouse CD25 PC61 FC
Brilliant Violet 750™ anti-mouse CD25 PC61 FC
PerCP/Fire™ 780 anti-mouse CD25 PC61 FC
PE/Fire™ 700 anti-mouse CD25 PC61 FC
Spark PLUS UV395™ anti-mouse CD25 PC61 FC
Spark Blue™ 574 anti-mouse CD25 (Flexi-Fluor™) PC61 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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