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Purified anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
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Product Citations
publications
PC61_Pure_091907
C57BL/6 mouse splenocytes stained with purified PC61, followed by anti-rat IgG FITC
  • PC61_Pure_091907
    C57BL/6 mouse splenocytes stained with purified PC61, followed by anti-rat IgG FITC
  • PC61_pure_CD25_Antibody_2_103018
    Fresh, frozen mouse spleen was stained with purified CD25 clone PC61 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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102002 500 µg £77
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Block, Depletion - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Ma F, et al. 2020. Theranostics. 5.784722222. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  4. Kondo H, et al. 2020. J Biol Chem. 295:1658. PubMed
  5. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  6. Quintana E, et al. 2020. Cancer Res. 80:2889. PubMed
  7. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  8. Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed
  9. Do DC, et al. 2019. JCI Insight. 4:e126832. PubMed
  10. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
  11. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  12. Liu F, et al. 2017. Front Immunol. 8:414. PubMed
  13. Gottschalk R, et al. 2012. Proc Natl Acad Sci U S A. 109:881. PubMed
  14. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  15. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  16. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
  17. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  18. Tan W, et al. 2021. Front Immunol. 12:653803. PubMed
  19. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  20. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  21. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  22. Kamio K, et al. 2018. Respir Res. 0.840972222. PubMed
  23. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  24. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  25. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  26. Anguela X, et al. 2013. Diabetes. 62:551. PubMed
  27. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  28. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  29. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  30. Murphy SF, et al. 2017. Pain. 158:1517. PubMed
  31. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  32. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  33. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  34. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  35. Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed
  36. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  37. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  38. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID
AB_312851 (BioLegend Cat. No. 102002)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Other Formats

View All CD25 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD25 PC61 FC
Biotin anti-mouse CD25 PC61 FC
FITC anti-mouse CD25 PC61 FC
PE anti-mouse CD25 PC61 FC
PE/Cyanine5 anti-mouse CD25 PC61 FC
Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
PE/Cyanine7 anti-mouse CD25 PC61 FC
Alexa Fluor® 488 anti-mouse CD25 PC61 FC
Alexa Fluor® 647 anti-mouse CD25 PC61 FC
Pacific Blue™ anti-mouse CD25 PC61 FC
Alexa Fluor® 700 anti-mouse CD25 PC61 FC
APC/Cyanine7 anti-mouse CD25 PC61 FC
PerCP/Cyanine5.5 anti-mouse CD25 PC61 FC
PerCP anti-mouse CD25 PC61 FC
Brilliant Violet 421™ anti-mouse CD25 PC61 FC
Brilliant Violet 605™ anti-mouse CD25 PC61 FC
Brilliant Violet 650™ anti-mouse CD25 PC61 FC
Ultra-LEAF™ Purified anti-mouse CD25 PC61 FC,IHC-F,IP,Block,Depletion
Brilliant Violet 510™ anti-mouse CD25 PC61 FC
PE/Dazzle™ 594 anti-mouse CD25 PC61 FC
Brilliant Violet 711™ anti-mouse CD25 PC61 FC
Brilliant Violet 785™ anti-mouse CD25 PC61 FC
Alexa Fluor® 594 anti-mouse CD25 PC61 IHC-F
APC/Fire™ 750 anti-mouse CD25 PC61 FC
TotalSeq™-A0097 anti-mouse CD25 PC61 PG
KIRAVIA Blue 520™ anti-mouse CD25 PC61 FC
TotalSeq™-B0097 anti-mouse CD25 PC61 PG
TotalSeq™-C0097 anti-mouse CD25 PC61 PG
Spark NIR™ 685 anti-mouse CD25 Antibody PC61 FC
PE/Fire™ 640 anti-mouse CD25 PC61 FC
Spark YG™ 581 anti-mouse CD25 PC61 FC
APC/Fire™ 810 anti-mouse CD25 PC61 FC
Brilliant Violet 750™ anti-mouse CD25 PC61 FC
PerCP/Fire™ 780 anti-mouse CD25 PC61 FC
PE/Fire™ 700 anti-mouse CD25 PC61 FC
Spark PLUS UV395™ anti-mouse CD25 PC61 FC
Spark Blue™ 574 anti-mouse CD25 (Flexi-Fluor™) PC61 FC
Go To Top Version: 2    Revision Date: 10/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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