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Brilliant Violet 421™ anti-human Ikaros Antibody

Pricing & Availability
Clone
16B5C71 (See other available formats)
Regulatory Status
RUO
Other Names
IK1, IKZF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
16B5C71_BV421_Ikaros_Antibody_1_022516
Human peripheral blood mononuclear cells were stained with CD19 Alex Fluor® 488, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom)
  • 16B5C71_BV421_Ikaros_Antibody_1_022516
    Human peripheral blood mononuclear cells were stained with CD19 Alex Fluor® 488, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom)
  • 16B5C71_BV421_Ikaros_Antibody_2_022516
Compare all formats See Brilliant Violet 421™ spectral data
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368405 25 tests £133
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368406 100 tests £298
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Description

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.


Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.

RRID
AB_2571956 (BioLegend Cat. No. 368405)
AB_2571956 (BioLegend Cat. No. 368406)

Antigen Details

Structure
Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution

Fetal and adult hematopoietic cells.

Function
Regulates lymphocyte differentiation.
Interaction
DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.

Gene ID
10320 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 02/29/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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