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Purified anti-human Ikaros Antibody

Pricing & Availability
Clone
16B5C71 (See other available formats)
Regulatory Status
RUO
Other Names
IK1, IKZF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
a-16B5C71_PURE_Ikaros_Antibody_WB_052316
Total lysates (15 µg protein) from Jurkat (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000) Purified anti-ikaros Antibody, clone 16B5C71 (upper), or 1:2000 diluted anti-GAPDH Antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-ikaros Antibody, or 1:3000 diluted donkey anti-Rabbit-IgG secondary antibody conjugated to HRP for anti-GAPDH Antibody. Lane M: Molecular weight ladder.
  • a-16B5C71_PURE_Ikaros_Antibody_WB_052316
    Total lysates (15 µg protein) from Jurkat (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000) Purified anti-ikaros Antibody, clone 16B5C71 (upper), or 1:2000 diluted anti-GAPDH Antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-ikaros Antibody, or 1:3000 diluted donkey anti-Rabbit-IgG secondary antibody conjugated to HRP for anti-GAPDH Antibody. Lane M: Molecular weight ladder.
  • b-16B5C71_GoChIP_Ikaros_Antibody_050918
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Jurkat cells. ChIP was performed with 5 µg of chromatin and either 4 µg of Go-ChIP-Grade™ purified anti-human Ikaros antibody (clone 16B5C71, Cat. No. 687104), or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (clone MG2a-53, Cat. No. 401506). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human Hes1 promoter region, and the human α-Satellite repeat element. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
  • c-16B5C71_GoChIP_Ikaros_Antibody_040220.png
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Jurkat cells. ChIP was performed with 5 µg of chromatin and either 4 µg of Go-ChIP-Grade™ purified anti-human Ikaros antibody (clone 16B5C71, Cat. No. 687104), or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (clone MG2a-53, Cat. No. 401506). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human Hes1 promoter region, and the human α-Satellite repeat element. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
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687102 100 µg £238
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Description

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. The recommended starting antibody to chromatin ratio is 4 µg of antibody and 5 µg of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.

RRID
AB_2616940 (BioLegend Cat. No. 687102)

Antigen Details

Structure
Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution

Fetal and adult hematopoietic cells.

Function
Regulates lymphocyte differentiation.
Interaction
DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.

Gene ID
10320 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org
Go To Top Version: 4    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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