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PerCP/Cyanine5.5 anti-human Ikaros Antibody

Pricing & Availability
Clone
16B5C71 (See other available formats)
Regulatory Status
RUO
Other Names
IK1, IKZF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
16B5C71_PerCPCyanine55_Ikaros_Antibody_1_061924
Human peripheral blood mononuclear cells were surface stained with anti-human CD19 (clone HIB19) Alexa Fluor® 488 and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then intracellularly stained with anti-human Ikaros (clone 16B5C71) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Data shown is gated on lymphocyte population.
  • 16B5C71_PerCPCyanine55_Ikaros_Antibody_1_061924
    Human peripheral blood mononuclear cells were surface stained with anti-human CD19 (clone HIB19) Alexa Fluor® 488 and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then intracellularly stained with anti-human Ikaros (clone 16B5C71) PerCP/Cyanine5.5 (left) or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Data shown is gated on lymphocyte population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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368415 25 tests £137
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368416 100 tests £310
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Description

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.

RRID
AB_3662272 (BioLegend Cat. No. 368415)
AB_3662272 (BioLegend Cat. No. 368416)

Antigen Details

Structure
Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution

Fetal and adult hematopoietic cells.

Function
Regulates lymphocyte differentiation.
Interaction
DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.

Gene ID
10320 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 06/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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