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Brilliant Violet 421™ anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
GL3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ
Isotype
Armenian Hamster IgG
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Product Citations
publications
GL3_BV421_1_051311
  • GL3_BV421_1_051311
  • GL3_BV421_2_051311
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
118119 125 µL £128
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118120 50 µg £163
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6J intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
  2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
  3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
  4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
  5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
  6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
  8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
  9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
Product Citations
  1. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  2. Schneider C, et al. 2018. Cell. 174:271. PubMed
  3. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  4. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  5. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  6. Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
  7. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  8. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  9. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  10. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  11. Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed
  12. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  13. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  14. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  15. Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed
  16. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  17. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  18. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  19. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  20. Jolly A, et al. 2022. Cell Rep Methods. 2:100315. PubMed
  21. Rezende RM, et al. 2019. J Immunol. 203:2621. PubMed
  22. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  23. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  24. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  25. Blanco R, et al. 2014. Sci Signal. 2:354. PubMed
RRID
AB_10896753 (BioLegend Cat. No. 118119)
AB_10896753 (BioLegend Cat. No. 118120)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex.
Distribution

T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.

Function
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand/Receptor
Some bacterial or tumor antigens bound to MHC class I.
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Skarstein K, et al. 1995. Immunology. 81:497.
  2. Harrison LC, et al. 1996. J Exp Med. 184:2167.
  3. Wildner G, et al. 1996. Eur J Immunol. 26:2140.
  4. Brandes M, et al. 2005. Science. 309:264.
Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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