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PerCP/Cyanine5.5 anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
GL3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ
Isotype
Armenian Hamster IgG
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Product Citations
publications
GL3_PerCPCyanine55_TCR-yS_Antibody_1_111924
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε FITC (clone 145-2C11) and anti-mouse TCR γ/δ (clone GL3) PerCP/Cyanine5.5 (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • GL3_PerCPCyanine55_TCR-yS_Antibody_1_111924
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε FITC (clone 145-2C11) and anti-mouse TCR γ/δ (clone GL3) PerCP/Cyanine5.5 (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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118117 25 µg £81
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118118 100 µg £221
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6J intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
  2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
  3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
  4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
  5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
  6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
  8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
  9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
Product Citations
  1. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  2. Polese B, et al. 2021. Cell Reports. 36(4):109456. PubMed
  3. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  4. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  5. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  6. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  7. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  8. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  9. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  10. Mikami Y, et al. 2021. Immunity. 54(3):514-525.e6. PubMed
  11. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  12. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  13. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  14. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  15. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  16. Wang Q, et al. 2020. Wound Repair Regen. 28:105. PubMed
  17. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  18. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  19. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  20. Babb R, et al. 2016. Clin Sci. 130: 697 - 710. PubMed
  21. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  22. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  23. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  24. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  25. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  26. Jin C, et al. 2019. Cell. 176:998. PubMed
  27. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  28. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  29. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  30. Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed
  31. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  32. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  33. Zhang H et al. 2019. Cell Rep. 29(2):391-405 . PubMed
  34. Mathur AN, et al. 2019. Immunity. 50:655. PubMed
  35. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  36. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  37. Wang X, et al. 2022. Elife. 11:. PubMed
  38. Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed
  39. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  40. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
RRID
AB_10612572 (BioLegend Cat. No. 118117)
AB_10612572 (BioLegend Cat. No. 118118)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex.
Distribution

T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.

Function
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand/Receptor
Some bacterial or tumor antigens bound to MHC class I.
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Skarstein K, et al. 1995. Immunology. 81:497.
  2. Harrison LC, et al. 1996. J Exp Med. 184:2167.
  3. Wildner G, et al. 1996. Eur J Immunol. 26:2140.
  4. Brandes M, et al. 2005. Science. 309:264.
Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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