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Brilliant Violet 650™ anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA18A28 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
Human peripheral blood mononuclear cells were surface stained with anti-human CD8a (clone RPA-T8) FITC. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with anti-human/mouse Granzyme B recombinant (clone QA18A28) Brilliant Violet 650™ (left) or rat IgG1, κ Brilliant Violet 650™ isotype control (right).
  • Human peripheral blood mononuclear cells were surface stained with anti-human CD8a (clone RPA-T8) FITC. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with anti-human/mouse Granzyme B recombinant (clone QA18A28) Brilliant Violet 650™ (left) or rat IgG1, κ Brilliant Violet 650™ isotype control (right).
Compare all formats See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
396435 25 tests £152
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396436 100 tests £298
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

RRID
AB_3662293 (BioLegend Cat. No. 396435)
AB_3662293 (BioLegend Cat. No. 396436)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
Neutrophils, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
  2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
  3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
  4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Other Formats

View All Granzyme B Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
FITC anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
APC anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
PE/Cyanine7 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Brilliant Violet 421™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
PerCP/Cyanine5.5 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Purified anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
PerCP anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
APC/Fire™ 750 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Pacific Blue™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Alexa Fluor® 647 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Alexa Fluor® 488 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Alexa Fluor® 700 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
PE/Dazzle™ 594 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
TotalSeq™-B1116 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICPG
Brilliant Violet 510™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Brilliant Violet 605™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Brilliant Violet 650™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Brilliant Violet 785™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Brilliant Violet 750™ anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Spark UV™ 387 anti-human/mouse Granzyme B Recombinant Antibody QA18A28 ICFC
Go To Top Version: 1    Revision Date: 03/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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