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- Clone
- QA18A28 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were stained with anti-human CD8 (clone RPA-T8) PE. Cells were then fixed, permeabilized, and intracellularly stained with anti-human/mouse Granzyme B (clone QA18A28) Brilliant Violet 750™ (left) or rat IgG1, κ Brilliant Violet 750™ isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 396439 | 25 tests | £152 | ||||
| 396440 | 100 tests | £298 | ||||
Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).
- RRID
-
AB_3662294 (BioLegend Cat. No. 396439)
AB_3662294 (BioLegend Cat. No. 396440)
Antigen Details
- Structure
- 32 kD serine protease
- Distribution
-
Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
- Function
- Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
- Interaction
- Caspase-3
- Ligand/Receptor
- Mannose-6-phosphate receptor
- Cell Type
- Neutrophils, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
-
- Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
- Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
- Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
- Wagner C, et al. 2008. Mol. Immunol. 45:1761.
- Gene ID
- 3002 View all products for this Gene ID 14939 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All Granzyme B Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... 
C57BL/6 mouse splenocytes were stimulated with plate-bound a... -
FITC anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
APC anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
PerCP/Cyanine5.5 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with C... -
Purified anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were fixed and perm... -
PerCP anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
APC/Fire™ 750 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Pacific Blue™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Alexa Fluor® 647 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Alexa Fluor® 488 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Alexa Fluor® 700 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
PE/Dazzle™ 594 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
TotalSeq™-B1116 anti-human/mouse Granzyme B Recombinant Antibody
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Brilliant Violet 510™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 605™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 650™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 785™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were surface staine... -
Brilliant Violet 750™ anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a... -
Spark UV™ 387 anti-human/mouse Granzyme B Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a...
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