- Clone
- N418 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- αX integrin, integrin αX chain, CR4, p150, ITGAX
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
117341 | 100 µg | £73 |
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse spleen dendritic cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Granucci F, et al. 1997. J. Immunol. 159:1794.
- Stokes RW, et al. 1998. J. Immunol. 160:5514.
- Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
- Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
- You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
- Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
- Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
- Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
- Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
- Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
- Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
- Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
- Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
- Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
- White CE, et al. 2015. J Immunol. 194:697. PubMed
- Lu X, et al. 2015. J Immunol. 194:2011. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2562807 (BioLegend Cat. No. 117341)
Antigen Details
- Structure
- Integrin α-chain, associates with integrin β2 (CD18), 150 kD
- Distribution
-
Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
- Function
- Cellular adhesion
- Ligand/Receptor
- iC3b, fibrinogen
- Cell Type
- Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780. - Gene ID
- 16411 View all products for this Gene ID
- UniProt
- View information about CD11c on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD11c Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 APC and PK136 PE -
Biotin anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... Frozen mouse spleen sections. Endogenous biotin blocking app... -
FITC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
PE anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Purified anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Alexa Fluor® 488 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Alexa Fluor® 647 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647 Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... Fixed whole mount mouse epidermis Langerhans cells were stai... Live intravital mouse spleen imaging. PE CD11b (red) (clone ... Confocal image of C57BL/6 mouse spleen sample acquired using... Fixed whole mount mouse spleen was stained with FITC CD172a ... -
PE/Cyanine5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
PE/Cyanine7 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Brilliant Violet 605™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and... -
Alexa Fluor® 700 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor®... -
Pacific Blue™ anti-mouse CD11c
C57BL/6 mouse splenocytes stained with Pacific Blue™ N... -
APC/Cyanine7 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and C... -
PerCP/Cyanine5.5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP/Cyanine5.5 -
PerCP anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP and M5/114... -
Brilliant Violet 421™ anti-mouse CD11c
C57BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10... C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FI... -
Brilliant Violet 570™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FI... -
Brilliant Violet 785™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and... -
Brilliant Violet 510™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E AP... -
Brilliant Violet 650™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E PE... -
Purified anti-mouse CD11c (Maxpar® Ready)
Mouse splenocytes stained with 147Sm-anti-CD45 (30-F11) and ... -
Alexa Fluor® 594 anti-mouse CD11c
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PE/Dazzle™ 594 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and... -
Brilliant Violet 711™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E Pe... -
APC/Fire™ 750 anti-mouse CD11c
C57BL/6 splenocytes were stained with I-A/I-E PE and CD11c (... -
TotalSeq™-A0106 anti-mouse CD11c
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Brilliant Violet 750™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
TotalSeq™-B0106 anti-mouse CD11c
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TotalSeq™-C0106 anti-mouse CD11c
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KIRAVIA Blue 520™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E APC and ... -
Spark Blue™ 550 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with I-A/I-E (clone M5/114... -
Spark NIR™ 685 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark UV™ 387 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark Red™ 718 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark Blue™ 515 anti-mouse CD11c
C57BL/6 mouse splenocytes were surface stained with anti-mou... -
PerCP/Fire™ 806 anti-mouse CD11c
C57BL/6 splenocytes were stained with anti-mouse I-A/I-E APC... -
Spark PLUS UV395™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark Blue™ 574 anti-mouse CD11c (Flexi-Fluor™)
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