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- Clone
- A16043E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SRK, STD, TZK, p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were treated with (left), or without (right), hydrogen peroxide for five minutes, fixed with Fixation Buffer (Cat No. 420801), permiabilized with Intracellular Staining Permeabilization Wash Buffer (Cat No. 421002), then intracellularly stained with CD3 Brilliant Violet 421™ and anti-ZAP70 Phospho (Tyr 493) PE (clone A16043E). -
Human peripheral blood lymphocytes were stimulated by CD3 and CD28 cross-linking (left) or unstimulated (right), fixed with Fixation Buffer (Cat. No. 420801), permiabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002), then intracellularly stained with CD3 Brilliant Violet 421™ and anti-ZAP70 Phospho (Tyr 493) (clone A16043E) PE. For CD3 and CD28 cross-linking, cells were incubated with Ultra-LEAF™ anti-CD3 (Cat. No. 300437) and LEAF™ anti-CD28 (Cat. No. 302914) on ice for 15 minutes followed by Purified anti-Mouse Ig (Cat. No. 405301) on ice for 15 minutes, and incubated at 37°C for 2 minutes.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 396003 | 25 tests | £105 | ||||
| 396004 | 100 tests | £247 | ||||
ZAP70 is a member of the Syk protein tyrosine kinase subfamily that is expressed exclusively in T cells and NK cells that plays an essential role in T cell receptor (TCR) signaling in combination with the Src family kinases, LCK and FYN. Upon TCR stimulation, phosphorylation of CD3 by LCK leads to the recruitment of ZAP70 to the activated receptor. Upon association of ZAP70 with the activated TCR complex, the kinase is phosphorylated on several tyrosine residues, including Tyrosine 493. Phosphorylation of Tyrosine 493, which is found within the activation loop of the protein, results in increased enzymatic activity. ZAP70 subsequently phosphorylates downstream targets, including the adaptor molecules LAT and LCP2, facilitating the recruitment and activation of other signaling molecules, ultimately leading to T cell proliferation, differentiation, and activation. ZAP70 mays also serve as a scaffold for recruiting additional factors to the activated TCR, and can function to regulate TCR-CD247/CD3Z expression at the plasma membrane. ZAP70 deficiency results in severely impaired immune function, while higher levels of ZAP70 expression are associated with certain B cell malignancies. PTN22 is required for the dephosphorylation of ZAP70 Tyrosine 493.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Positive staining is stimulation and donor dependent. Upon stimulation at high concentrations of H202 some staining of B cells can be seen. Staining for ZAP70 Phospho (Tyr493) using clone A16043E can also be successfully performed using the True-Nuclear™ Transcription Buffer Set and True-Phos™ Perm Buffer.
- RRID
-
AB_2801068 (BioLegend Cat. No. 396003)
AB_2801068 (BioLegend Cat. No. 396004)
Antigen Details
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
- Gene ID
- 7535 View all products for this Gene ID
- UniProt
- View information about ZAP70 Phospho Tyr493 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All ZAP70 Phospho (Tyr493) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human ZAP70 Phospho (Tyr493) | A16043E | ICFC |
| PE/Cyanine7 anti-human ZAP70 Phospho (Tyr493) | A16043E | ICFC |
| APC anti-human ZAP70 Phospho (Tyr493) | A16043E | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human ZAP70 Phospho (Tyr493)
Human peripheral blood lymphocytes were treated with (left),... 
Human peripheral blood lymphocytes were stimulated by CD3 an... -
PE/Cyanine7 anti-human ZAP70 Phospho (Tyr493)
Human peripheral blood lymphocytes were treated with (left),... -
APC anti-human ZAP70 Phospho (Tyr493)
Human peripheral blood lymphocytes were treated with (left),...
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