Purified anti-HMB-45 Antibody anti-HMB-45

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Clone: HMB-45 (See other available formats)
Regulatory Status: RUO
Other Names: Melanoma Marker antibody, Human Melanoma Black, Melanocyte protein PMEL, PMEL
Isotype: Mouse IgG1, κ
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HMB-45_PURE_HMB-45_Antibody_IHC_1_091117 — IHC staining of anti-HMB-45 antibody (clone HMB-45) on formalin-fixed paraffin-embedded human melanoma tissue. The tissue was incubated with anti-HMB-45 (top) or mouse IgG1 κ control (bottom) at 5µg/mL for 24 hours at 4°C. Fast Red was used for detection followed by hematoxylin counterstaining.

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911503

25 µg

£85

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911504

100 µg

£213

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Description

The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2716121 (BioLegend Cat. No. 911503)
AB_2716121 (BioLegend Cat. No. 911504)

Antigen Details

Structure: HMB-45 (Human Melanoma Black) is a monoclonal antibody that reacts with an antigen known as Pmel 17 present in melanocytic tumors such as melanomas.
Distribution: Tissue: expressed mainly in pigment cells of the skin, hair and eyes.
Distribution: melanocytes, intracellular.
Gene ID: 6490 View all products for this Gene ID
UniProt: View information about HMB-45 on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Description	Clone	Applications

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Other Formats

View All HMB-45 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-HMB-45	HMB-45	IHC-P,IHC-F
Alexa Fluor® 647 anti-HMB-45	HMB-45	IHC-P
Alexa Fluor® 594 anti-HMB-45	HMB-45	IHC-P
Alexa Fluor® 488 anti-HMB-45	HMB-45	IHC-P
Spark YG™ 570 anti-HMB-45	HMB-45	IHC-P

Certificate of Analysis

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Go To Top Version: 0 Revision Date: 09/11/2017

For Research Use Only. Not for diagnostic or therapeutic use.

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