- Clone
- SK3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T4, Leu3a
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![SK3_Purified_CD4_Cytof_060414.jpg SK3_Purified_CD4_Cytof_060414.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SK3_Purified_CD4_Cytof_060414.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 174Yb-anti-CD4 (SK3). Data provided by DVS Sciences.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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344625 | 100 µg | £117 |
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Evans RL, et al. 1981. Immunol. 78:544
- Arno A et al. 1999. J. Infect. Dis. 180:56
- Muech M, et al. 1997. Blood 89:1364
- Wang L, et al. 2012. Cytometry A. 81:567. PubMed
- Product Citations
-
- RRID
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AB_2563749 (BioLegend Cat. No. 344625)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 55 kD
- Distribution
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T cell subset, majority of thymocytes, monocytes/macrophages
- Function
- MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
- Ligand/Receptor
- MHC class II molecules, HIV gp120, IL-16
- Cell Type
- Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. - Gene ID
- 920 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
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We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PerCP anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Purified anti-human CD4
Human peripheral blood lymphocytes stained with SK3, followe... -
FITC anti-human CD4
Human peripheral blood lymphocytes stained with SK3 FITC -
PE anti-human CD4
Human peripheral blood lymphocytes stained with SK3 PE -
PerCP/Cyanine5.5 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
Biotin anti-human CD4
Human peripheral blood lymphocytes stained with biotinylated... -
PE/Cyanine7 anti-human CD4
Human peripheral blood lymphocytes stained with SK3 PE/Cyani... -
APC anti-human CD4
Human peripheral blood lymphocytes stained with SK3 APC -
APC/Cyanine7 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 488 anti-human CD4
Human peripheral blood lymphocytes stained with SK3 Alexa Fl... -
Pacific Blue™ anti-human CD4
Human peripheral blood lymphocytes stained with SK3 Pacific ... -
Alexa Fluor® 700 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Purified anti-human CD4 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 174Yb-an... -
PE anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 421™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 510™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Alexa Fluor® 647 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
FITC anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 PE ... -
PE/Dazzle™ 594 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Pacific Blue™ anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 785™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 750™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 605™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 711™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Cyanine7 anti-human CD4
Typical results from human peripheral blood lymphocytes sta... -
TotalSeq™-A0045 anti-human CD4
-
TotalSeq™-C0045 anti-human CD4
-
PE/Cyanine5 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 550 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (SK... -
Spark NIR™ 685 anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 (cl... -
APC anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
KIRAVIA Blue 520™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 APC... -
APC/Fire™ 810 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Fire™ 640 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Fire™ 700 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 538 anti-human CD4 Antibody
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Spark YG™ 581 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
Alexa Fluor® 660 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Spark YG™ 593 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD4 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 750 anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 574 anti-human CD4
Human peripheral lymphocytes were stained with anti-human CD... -
GMP APC anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-B0045 anti-human CD4 Antibody
-
Spark Violet™ 423 anti-human CD4 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP Pacific Blue™ anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 500 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP/Cyanine5.5 anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 650™ anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 806 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 515 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP APC/Fire™ 750 anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human CD4
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Fire™ 780 anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 744 anti-human CD4
Human peripheral blood mononuclear cells were stained with a... -
Spark PLUS UV™ 395 anti-human CD4
Human peripheral blood lymphocytes were stained anti-human C... -
Spark PLUS B550™ anti-human CD4
Human peripheral blood lymphocytes were stained with anti-hu...
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