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Purified anti-human CD49c (integrin α3) Antibody

Pricing & Availability
Clone
ASC-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI A002
Other Names
α3 integrin, integrin α3 chain, VLA-3 α chain, VLA3a, ITGA3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ASC-1_PE_082509
Human cervical cancer cell line HeLa cells stained with purified ASC-1 conjugated to PE
  • ASC-1_PE_082509
    Human cervical cancer cell line HeLa cells stained with purified ASC-1 conjugated to PE
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343802 100 µg £94
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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human squamous cell carcinoma cell line SCC-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation4, Western blotting5, and immunohistochemical staining of frozen tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Pattaramalai S, et al. 1996. Exp. Cell. Res. 222:281.
  2. Skubitz AP, et al. 1996. Am. J. Pathol. 148:1445. (IHC)
  3. Skubitz AP, et al. 1998. FEBS Lett. 426:386.
  4. Calzada MJ, et al. 2004. Circ. Res. 94:462. (IP)
  5. Zhang X, et al. 2003. Surgery. 133:429. (WB)
Product Citations
  1. Cegarra C, et al. 2022. PLoS One. 17:e0274667. PubMed
RRID
AB_1731901 (BioLegend Cat. No. 343802)

Antigen Details

Structure
150 kD type I transmembrane glycoprotein, integrin family, associate with integrin β1(CD29)
Distribution

Endothelial cells, epithelial cells, dermal fibroblast

Function
adhesion
Ligand/Receptor
Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. John Wiley & Sons Inc.

Gene ID
3675 View all products for this Gene ID
UniProt
View information about CD49c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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