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Purified anti-mouse CD103 Antibody

Pricing & Availability
Clone
2E7 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Armenian Hamster IgG
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Product Citations
publications
2E7_Purified_071006
C57BL/6 mouse splenocytes stained with PE CD3 (clone 17A2) and purified 2E7, followed by anti-Armenian hamster IgG FITC
  • 2E7_Purified_071006
    C57BL/6 mouse splenocytes stained with PE CD3 (clone 17A2) and purified 2E7, followed by anti-Armenian hamster IgG FITC
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121401 50 µg £57
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121402 200 µg £129
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Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  3. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  4. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  5. Feriotti C, et al. 2022. Cell Rep. 40:111167. PubMed
  6. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  7. Mikami N, et al. 2011. J Immunol. 186:6886. PubMed
  8. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  9. Tian D, et al. 2020. FASEB J. 34:3367. PubMed
  10. Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed
  11. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  12. Li W, et al. 2020. J Clin Invest. 130:6718. PubMed
  13. Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed
  14. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  15. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  16. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  17. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  18. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  19. Blriot C, et al. 2021. Immunity. :. PubMed
  20. Yao Y, et al. 2017. Nat Commun. 8:15402. PubMed
  21. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  22. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  23. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  24. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  25. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  26. Vander B, et al. 2017. J Cell Biol. 10.1083/jcb.201512012. PubMed
  27. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  28. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  29. Maier B, et al. 2020. Nature. 580:257. PubMed
  30. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID
AB_535944 (BioLegend Cat. No. 121401)
AB_535944 (BioLegend Cat. No. 121402)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/04/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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