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- Clone
- 4G9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transitional endoplasmic reticulum ATPase, p97, TERA
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 636801 | 25 µg | £77 | ||||
| 636802 | 100 µg | £157 | ||||
p97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97/VCP protein.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human valosin containing protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
IP, ICC - Reported by the developer, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 mL antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2214641 (BioLegend Cat. No. 636801)
AB_2214641 (BioLegend Cat. No. 636802)
Antigen Details
- Structure
- Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
- Interaction
- VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP
- Bioactivity
- VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Endoplasmic Reticulum Markers
- Antigen References
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1. Ye Y, et al. 2001. Nature 414:652.
- Gene ID
- 7415 View all products for this Gene ID
- UniProt
- View information about p97/VCP on UniProt.org
Other Formats
View All p97/VCP Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-p97/VCP | 4G9 | WB,IP,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-p97/VCP
Western blot analysis of extracts from Hela cells (Lane 1), ...
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