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Purified anti-SOX2 (NH2 terminus) Antibody

Pricing & Availability
Clone
Poly6308 (See other available formats)
Regulatory Status
RUO
Other Names
SRY (sex determining region Y)-box 2, ANOP3, SOX-2
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6308_PURE_SOX2_Antibody_WB_060418
Total lysates (15 µg protein) from NTERA-2 and HeLa were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 purified anti-SOX2 antibody, clone Poly6308. Proteins were visualized using chemiluminescence detection by incubation with HRP anti-Rabbit secondary antibody (Cat. No. 410406, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • Poly6308_PURE_SOX2_Antibody_WB_060418
    Total lysates (15 µg protein) from NTERA-2 and HeLa were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 purified anti-SOX2 antibody, clone Poly6308. Proteins were visualized using chemiluminescence detection by incubation with HRP anti-Rabbit secondary antibody (Cat. No. 410406, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
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630801 50 µL £101
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630802 200 µL £221
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Description

This rabbit polyclonal antibody recognizes human SOX2 also known as SRY (sex determining region Y)-box 2 and ANOP3. SOX2 is a nucleus protein, predicted molecular weight 34 kD. SOX2 belongs to the SOX (SRY-box containing gene) gene family which encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. Defects in SOX2 are a cause of true or primary anophthalmos, also called anophthalmia or ANOP3. Anophthalmos is a developmental defect characterized by complete absence of the eyes (rare) or by the presence of vestigial eyes. The poly6308 antibody has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH (N-terminal)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each minigel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Product Citations
  1. Zywitza V, et al. 2022. iScience. 25:105414. PubMed
  2. Zhou T et al. 2017. Cell stem cell. 21(2):274-283 . PubMed
  3. Tchieu J et al. 2017. Cell stem cell. 21(3):399-410 . PubMed
  4. Rossbach B et al. 2016. Stem cell research. 16(2):314-7 . PubMed
  5. Ouyang J, et al. 2015. J Biol Chem. 290: 22782-22794. PubMed
  6. Cederquist GY, et al. 2020. Cell Stem Cell. 27(1):35-49.e6. PubMed
  7. Fu S, et al. 2018. Int J Mol Med. 41:87. PubMed
RRID
AB_2195784 (BioLegend Cat. No. 630801)
AB_2195784 (BioLegend Cat. No. 630802)

Antigen Details

Structure
Belongs to the SOX (SRY-box containing gene) gene family, predicted molecular weight 34 kD.
Distribution

Nucleus protein

Function
The SOX gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate.
Interaction
Interacts with OCT4, OCT1, FTZ1, and Fibroblast growth factor 4 proteins.
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References

1. Stevanovic M, et al. 1994. Mammalian Genome 5:640.
2. Okubo T, et al. 2006. Genes Dev. 20:2654.
3. Ragge NK, et al. 2005. Am. J. Med. Genet. 135A:1.

Gene ID
6657 View all products for this Gene ID
UniProt
View information about SOX2 on UniProt.org
Go To Top Version: 4    Revision Date: 05/04/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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