Ultra-LEAF Purified anti-human CD11b Antibody, CD11b, ICRF44

Pricing & Availability

Clone: ICRF44 (See other available formats)
Regulatory Status: RUO
Workshop: IV M047
Other Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype: Mouse IgG1, κ
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Product Citations: publications

ICRF44_LEAF_061407 — Human peripheral blood granulocytes stained with purified ICRF44, followed by anti-mouse IgGs FITC

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301361

100 µg

£185

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301362

1 mg

£464

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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as α_M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β₂ (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Chimpanzee, Common Marmoset, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
CyTOF® - Verified
Block, ICC, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).
RRID: AB_2814118 (BioLegend Cat. No. 301361)
AB_2814118 (BioLegend Cat. No. 301362)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
Gene ID: 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD11b Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD11b	ICRF44	FC
Biotin anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
PE/Cyanine5 anti-human CD11b	ICRF44	FC
Purified anti-human CD11b	ICRF44	FC,CyTOF®,ICC,IHC
Pacific Blue™ anti-human CD11b	ICRF44	FC
Alexa Fluor® 488 anti-human CD11b	ICRF44	FC
Alexa Fluor® 647 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Brilliant Violet 421™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 570™ anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
Brilliant Violet 605™ anti-human CD11b	ICRF44	FC
Brilliant Violet 510™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 650™ anti-human CD11b	ICRF44	FC
Purified anti-human CD11b (Maxpar® Ready)	ICRF44	FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b	ICRF44	ICC,FC
APC/Cyanine7 anti-human CD11b	ICRF44	FC
Brilliant Violet 711™ anti-human CD11b	ICRF44	FC
Brilliant Violet 785™ anti-human CD11b	ICRF44	FC
PE/Dazzle™ 594 anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
APC anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
TotalSeq™-A0161 anti-human CD11b	ICRF44	PG
Alexa Fluor® 700 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
TotalSeq™-B0161 anti-human CD11b	ICRF44	PG
TotalSeq™-C0161 anti-human CD11b	ICRF44	PG
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Ultra-LEAF™ Purified anti-human CD11b	ICRF44	FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b	ICRF44	PG
GMP PE/Cyanine7 anti-human CD11b	ICRF44	FC
Pacific Blue™ anti-human CD11b	ICRF44	FC
GMP PE anti-human CD11b	ICRF44	FC
Spark UV™ 387 anti-human CD11b	ICRF44	FC
GMP PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
GMP APC anti-human CD11b	ICRF44	FC
GMP FITC anti-human CD11b	ICRF44	FC
GMP APC/Fire™ 750 anti-human CD11b	ICRF44	FC
Spark Blue™ 515 anti-human CD11b	ICRF44	FC
Spark Violet™ 500 anti-human CD11b	ICRF44	FC
Spark Red™ 718 anti-human CD11b (Flexi-Fluor™)	ICRF44	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 01/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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