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• Testing on multiple cell and tissue types with a variety of known expression levels.
• Validation in multiple applications as a cross-check for specificity and to provide additional clarity for researchers.
• Comparison to existing antibody clones.
• Using cell treatments to modulate target expression, such as phosphatase treatment to ensure phospho-antibody specificity.

Testing on Multiple Cell Lines

Testing in Multiple Applications

One of the most trusted ways to ensure specificity is to use knockdown or knockout models. This is especially true for ubiquitously expressed proteins where there aren’t negative control or low expressing cell types available. In these cases, we also use either siRNA-mediated knockdown or CRISPR/Cas9-mediated knockout of the target protein.

Western blot of total lysates from HeLa and HeLa AKT1CRISPR/Cas9 knockout (KO) cells probed with anti-AKT1 (clone O94E10).

Check out our updated our Stem Cells and Development wall poster incorporating the latest in stem cell biology including development into the three major germ layers, induced pluripotent stem cells, and how the niche affects stem cell development. Visit our Stem Cell Poster Webpage to view products to study stem cells, download the poster, or request a physical copy to hang in the lab.