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Alexa Fluor® 700 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_Alx700_080408.jpg
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
  • UCHT1_Alx700_080408.jpg
    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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300423 25 µg $126
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300424 100 µg $252
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kim JH, et al. 2020. Sci Rep. 10:1835. PubMed
  2. Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed
  3. Labuz DR, et al. 2023. Elife. 12: . PubMed
  4. Laprevotte E, et al. 2013. Leuk Res. 37:440. PubMed
  5. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  6. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  7. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  8. Xiong H, et al. 2023. EBioMedicine. 90:104507. PubMed
  9. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
  10. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed
  11. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  12. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  13. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  14. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  15. Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed
  16. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  17. Keustermans G,et al. 2017. PLoS One. 10.1371/journal.pone.0187068. PubMed
  18. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  19. Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed
  20. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
  21. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  22. Na I, et al. 2013. Haematologica. 98:23. PubMed
  23. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  24. Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed
  25. Kiniry B, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.100. PubMed
  26. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  27. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  28. Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
  29. Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
  30. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  31. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  32. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  33. Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed
  34. Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed
  35. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  36. Bochem J, et al. 2019. PLoS One. 14:e0221301. PubMed
  37. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  38. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  39. Yoshida R, et al. 2022. Cancer Res. :. PubMed
  40. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  41. Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
  42. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  43. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  44. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  45. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  46. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  47. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  48. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  49. Hood SP, et al. 2020. Elife. 9:00. PubMed
  50. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  51. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  52. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  53. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
  54. Goc J, et al. 2021. Cell. 184:5015. PubMed
  55. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  56. Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed
  57. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
RRID
AB_493740 (BioLegend Cat. No. 300423)
AB_493740 (BioLegend Cat. No. 300424)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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