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APC/Fire™ 750 anti-human CD11c Antibody

Pricing & Availability
Clone
S-HCL-3 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αX subunit, CR4, p150, ITGAX
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
S-HCL-3_APC-Fire750_CD11c_Antibody_FC_012517
Human peripheral blood granulocytes were stained with CD11c (clone S-HCL-3) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
  • S-HCL-3_APC-Fire750_CD11c_Antibody_FC_012517
    Human peripheral blood granulocytes were stained with CD11c (clone S-HCL-3) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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371509 25 tests $171
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371510 100 tests $382
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Description

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αx and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Spleen cells from patient diagnosed with hairy cell leukemia.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1,2,3,4 and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Schwarting R, et al. 1985. Blood 65:974.
  2. Knowles DM, et al. 1990. Am. J. Pathol. 136:29.
  3. Vandenabeele S, et al. 2001. Blood 97:1733.
  4. Shaw JL, et al. 2011. J. Reprod. Immunol. 89:84.
Product Citations
  1. Pizzolla A, et al. 2021. Methods Mol Biol. 2265:529. PubMed
  2. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  3. Asokan M, et al. 2020. Proc Natl Acad Sci U S A. 117:18754. PubMed
  4. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  5. Woodruff M, et al. 2020. medRxiv. . PubMed
  6. Parker R, et al. 2021. Cell Reports. 35(8):109179. PubMed
RRID
AB_2650792 (BioLegend Cat. No. 371509)
AB_2650792 (BioLegend Cat. No. 371510)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin ß2 (CD18), 145-150 kD.
Distribution

Myeloid, dendritic cells, NK cells, B cells and T cell subsets.

Function
Adhesion and CTL killing.
Interaction
Interacts with fibrinogen, CD54, and iC3b.
Ligand/Receptor
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Petty HR, Todd RF 3rd. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-10.

Gene ID
3687 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD11c
Specificity Alt (DOES NOT SHOW ON TDS):
CD11c
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD11c on UniProt.org
Go To Top Version: 1    Revision Date: 01/30/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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