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PE anti-mouse/human CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
DECMA-1 (See other available formats)
Regulatory Status
RUO
Other Names
E-Cadherin, Cadherin-1, CDH1, and UVO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
DECMA_PE_CD324_Antibody_FC_011714
MDCK epithelial cell line was stained with CD324 (clone DECMA-1) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).
  • DECMA_PE_CD324_Antibody_FC_011714
    MDCK epithelial cell line was stained with CD324 (clone DECMA-1) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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147303 25 µg $153
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147304 100 µg $335
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Description

CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cynomolgus, Dog, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E-Cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, biological function1,2, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
  2. Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
  3. Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  2. Bracey NA, et al. 2021. Life Sci Alliance. 4:00. PubMed
  3. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  4. Little DR, et al. 2021. Nat Commun. 12:2509. PubMed
  5. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  6. Zhang X, et al. 2019. iScience. 19:607. PubMed
  7. Zhang J, et al. 2018. Cell Death Dis. 9:224. PubMed
  8. Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed
  9. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  10. Schwarz BA et al. 2018. Cell stem cell. 23(2):289-305 . PubMed
RRID
AB_2563039 (BioLegend Cat. No. 147303)
AB_2563039 (BioLegend Cat. No. 147304)

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor
αEβ7 integrin.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.

Gene ID
999 View all products for this Gene ID 12550 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD324
Specificity Alt (DOES NOT SHOW ON TDS):
CD324
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD324 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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