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Alexa Fluor® 594 anti-mouse/human CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
DECMA-1 (See other available formats)
Regulatory Status
RUO
Other Names
E-Cadherin, Cadherin-1, CDH1, and UVO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
1_DECMA-1_AF594_CD324_Antibody_ICC_051519
Madin-darby canine kidney epithelial cell line, MDCK, was cultured in a chamber slide until confluent. The cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. The cells were then intracellularly stained with 10 µg/mL of CD324 (clone DECMA-1) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 1_DECMA-1_AF594_CD324_Antibody_ICC_051519
    Madin-darby canine kidney epithelial cell line, MDCK, was cultured in a chamber slide until confluent. The cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. The cells were then intracellularly stained with 10 µg/mL of CD324 (clone DECMA-1) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 2_DECMA-1_A594_CD324_Antibody_IHC-P_020818
    Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD324 (clone DECMA-1) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • 3_DECMA-1_AF594_CD324_Antibody_IHCF_051519
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/mL of CD324 (clone DECMA-1) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 4_DECMA-1_A594_CD324_Antibody_3DIHC_052621
    Paraformaldehyde-fixed (1%), 500 μm-thick mouse liver tissue section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD324 (E-Cadherin) antibody (clone DECMA-1) Alexa Fluor® 594 at 5 μg/mL (yellow), and anti-mouse CLEC4F antibody (clone 3E3F9) Alexa Fluor® 647 at 5 μg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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147306 100 µg $206
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Description

CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cynomolgus, Dog, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E-Cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P, IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 20 μg/mL is recommended. For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 μg/mL is suggested. For immunohistochemical staining of frozen tissue sections, a concentration range of 2.0 - 5.0 μg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, biological function1,2, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
  2. Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
  3. Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  2. Huber M, et al. 2023. Nat Commun. 14:2143. PubMed
  3. Zhang X, et al. 2019. iScience. 19:607. PubMed
  4. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
RRID
AB_2563230 (BioLegend Cat. No. 147306)

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor
αEβ7 integrin.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.

Gene ID
999 View all products for this Gene ID 12550 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org
Go To Top Version: 3    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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