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PerCP/Cyanine5.5 anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
GL7_PerCPCyanine55_GL7_Antibody_1_031324
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7) PerCP/Cyanine5.5 (left) or rat IgM, κ PerCP/Cyanine5.5 isotype control (right).
  • GL7_PerCPCyanine55_GL7_Antibody_1_031324
    BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7) PerCP/Cyanine5.5 (left) or rat IgM, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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144609 25 µg $176
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144610 100 µg $382
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
  2. Chaurio RA, et al. 2022. Immunity. 55:115. PubMed
  3. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  4. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  5. Leung W, et al. 2022. Cancer Discov. 12:1782. PubMed
  6. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  7. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  8. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  9. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  10. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  11. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  12. Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
  13. Suzuki Y, et al. 2022. Mol Ther Nucleic Acids. 30:226. PubMed
  14. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  15. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  16. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  17. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  18. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  19. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  20. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  21. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  22. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  23. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  24. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  25. Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
  26. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  27. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  28. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  29. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  30. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  31. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  32. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  33. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  34. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
  35. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  36. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
  37. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
RRID
AB_2562978 (BioLegend Cat. No. 144609)
AB_2562978 (BioLegend Cat. No. 144610)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
GL7
Specificity Alt (DOES NOT SHOW ON TDS):
GL7
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about GL7 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 01/08/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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