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Purified anti-β-Tubulin Antibody

Pricing & Availability
Clone
O95C1 (See other available formats)
Regulatory Status
RUO
Other Names
Tubulin 5 β, Tubulin β-4 chain
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O95C1_PURE_beta-Tubulin_Antibody_WB_012618_updated
Whole cell extracts (15 µg total protein) prepared from HeLa (Lane 1), Jurkat (Lane 2), NTERA-2 (lane 3), and NIH/3T3 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-β-Tubulin antibody (Clone O95C1). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301). Equal protein loading was confirmed using Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) at a 1:5000 dilution (lower). Lane M: MW ladder.
  • O95C1_PURE_beta-Tubulin_Antibody_WB_012618_updated
    Whole cell extracts (15 µg total protein) prepared from HeLa (Lane 1), Jurkat (Lane 2), NTERA-2 (lane 3), and NIH/3T3 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-β-Tubulin antibody (Clone O95C1). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301). Equal protein loading was confirmed using Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) at a 1:5000 dilution (lower). Lane M: MW ladder.
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605101 25 µg $101
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605102 100 µg $253
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Description

Eukaryotic microtubules are tubular polymers of tubulin that are a major component of the cytoskeleton. They play an essential role in diverse cellular functions, including migration, mitosis, and trafficking, and are also critical for normal development. β- and α-tubulins comprise the two core protein families that heterodimerize and assemble into each microtubule filament. There are multiple β- and α-tubulins isotypes, with each showing unique tumoral and tissue-specific distribution. TUBB4A is a β–tubulin primarily expressed in nervous tissue and moderate expression in testis. Mutations in TUBB4A cause hypermyelinating leukodystrophy-6 and autosomal dominant torsion dystonia -4.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length protein expressed in HEK293T cells transfected with human TUBB4 expression vector.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Because of complete sequence homology, this clone is also predicted to recognize TUBB4A in higher eukaryotes, including rat, monkey, pig, bovine, and canine. This clone was generated using full-length recombinant TUBB4A as an immunogen. However, due to high sequence homology, this clone may also recognize other members of the ß-Tubulin family, notably TUBB2A, TUBB3, TUBB4B, and TUBB8. Because these ß-Tubulins have a similar molecular weight as TUBB4A, we do not recommend using this antibody as a TUBB4A-specific antibody. This clone was generated using a more defined immunogen (recombinant TUBB4A) than our existing ß-Tubulin clone, TU27/Tubulin (Cat. No. 903401), which was generated against taxol-assembled microtubules (mixed tubulins).

Product Citations
  1. Ferreira D, et al. 2019. Sci Rep. 9:3958. PubMed
RRID
AB_2728462 (BioLegend Cat. No. 605101)
AB_2728462 (BioLegend Cat. No. 605102)

Antigen Details

Structure
TUBB4A is a 444 amino acid protein with a predicted molecular weight of 49.58 kD.
Distribution

β-Tubulins are ubiquitously expressed, with TUBB4A showing highest expression levels in brain and moderate expression in testis. TUBB4A shows low expression in other tissues.

Function
Cell migration, cell trafficking, mitosis, cilia and flagella motor dynamics.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References
  1. Arai-Ichinoi N, et al. 2016. Hum. Genet. 135: 89.
  2. Romaniello R, et al. 2015. Brain Dev. 37: 273.
  3. Shimojima K, et al. 2015. Brain Dev. 37: 281.
  4. Pizzino A, et al. 2014. Neurology. 83: 898.
  5. Tamura D, et al. 2013. Cancer Med. 2: 144.
  6. Hersheson J, et al. 2013. Ann. Neurol. 73: 546.
Gene ID
10382 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
beta-Tubulin
Specificity Alt (DOES NOT SHOW ON TDS):
β-Tubulin
App Abbreviation (DOES NOT SHOW ON TDS):
WB
UniProt
View information about beta-Tubulin on UniProt.org
Go To Top Version: 2    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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