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Alexa Fluor® 488 anti-mouse CD11a Antibody

Pricing & Availability
Clone
I21/7 (See other available formats)
Regulatory Status
RUO
Other Names
αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL,
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
I21slash7_A488_CD11a_Antibody_081117
C57BL/6 mouse splenocytes were stained with CD11a (clone I21/7) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control Alexa Fluor® 488 (open histogram).
  • I21slash7_A488_CD11a_Antibody_081117
    C57BL/6 mouse splenocytes were stained with CD11a (clone I21/7) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control Alexa Fluor® 488 (open histogram).
Compare all formats See Alexa Fluor® 488 spectral data
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153105 25 µg $101
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153106 100 µg $223
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Description

CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Whole T cells Lymphoma BW5147 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1981. J. Exp. Med. 154:1517.
  2. Champe M, et al. 1995. J. Biochem. 270:1388.
Product Citations
  1. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
RRID
AB_2716035 (BioLegend Cat. No. 153105)
AB_2716035 (BioLegend Cat. No. 153106)

Antigen Details

Structure
Integrin, associated with integrin CD18, 180 kD
Distribution

Lymphocytes, granulocytes, monocytes, macrophages

Function
Intercellular adhesion
Interaction
Associates with CD18
Ligand/Receptor
ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50)
Cell Type
Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.

Gene ID
16408 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org
Go To Top Version: 1    Revision Date: 08/14/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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