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Alexa Fluor® 488 anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
390_Alx488_080607
C57BL/6 mouse splenocytes stained with 390 Alexa Fluor® 488
  • 390_Alx488_080607
    C57BL/6 mouse splenocytes stained with 390 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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102413 25 µg $101
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102414 100 µg $229
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Pernet E, et al. 2023. Nature. 614:530. PubMed
  2. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  3. Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed
  4. Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
  5. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
  6. Watanabe S, et al. 2015. J Virol. 89:5847. PubMed
  7. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  8. Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
  9. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  10. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  11. Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed
  12. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  13. Melhem H, et al. 2022. Mucosal Immunol. 15:443. PubMed
  14. Kirkby N, et al. 2012. Proc Natl Acad Sci U S A. 109:17597. PubMed
  15. Fan Z, et al. 2021. Cell Metabolism. 33:1793. PubMed
  16. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  17. Kazakevych J, et al. 2019. Sci Rep. 9:10410. PubMed
  18. Losenkova K, et al. 2020. J Cell Sci. 133:00:00. PubMed
  19. Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed
  20. Lupan AM, et al. 2021. Aging (Albany NY). 13:15875. PubMed
  21. Namachivayam K, et al. 2020. Proc Natl Acad Sci U S A. 117:10958. PubMed
  22. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
RRID
AB_493408 (BioLegend Cat. No. 102413)
AB_493408 (BioLegend Cat. No. 102414)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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