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Brilliant Violet 421™ anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
390_BV421_CD31_Antibody_FC_061013
C57BL/6 mouse splenocytes were stained with CD31 (clone 390) Brilliant Violet 421™ (open histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 390_BV421_CD31_Antibody_FC_061013
    C57BL/6 mouse splenocytes were stained with CD31 (clone 390) Brilliant Violet 421™ (open histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
102423 125 µL $182
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102424 50 µg $273
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  2. Dokun A, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 790-803. PubMed
  3. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  4. Dong W, et al. 2022. Theranostics. 12:4110. PubMed
  5. Alexandre YO, et al. 2022. STAR Protoc. 3:101923. PubMed
  6. Huang Z, et al. 2022. Cell Rep. 39:110575. PubMed
  7. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  8. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  9. Kim SS, et al. 2022. Cancers (Basel). 14:. PubMed
  10. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  11. Kugler J, et al. 2015. Toxicol Sci. 146: 374-385. PubMed
  12. Quaife-Ryan GA, et al. 2020. Development. 147: . PubMed
  13. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  14. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  15. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  16. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  17. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  18. Redpath AN, et al. 2021. STAR Protocols. 2(1):100359. PubMed
  19. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  20. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  21. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  22. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  23. Chuang CH, et al. 2020. Cancer Research. 81:567. PubMed
  24. Ayari A, et al. 2020. Commun Biol. 3:237. PubMed
  25. Ma Z, et al. 2022. Methods Mol Biol. 2471:49. PubMed
  26. Wakabayashi T, et al. 2022. Invest Ophthalmol Vis Sci. 63:5. PubMed
  27. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  28. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  29. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  30. Dong W, et al. 2020. Sci Rep. 10:6652. PubMed
  31. Ombrato L, et al. 2021. Nat Protoc. 16:872. PubMed
  32. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  33. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
  34. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  35. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  36. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  37. Quaife-Ryan GA, et al. 2020. Development. . PubMed
RRID
AB_2562186 (BioLegend Cat. No. 102423)
AB_2562186 (BioLegend Cat. No. 102424)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 03/29/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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