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Alexa Fluor® 647 anti-mouse CD133 Antibody

Pricing & Availability
Clone
315-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
Prominin-1, AC133
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
315-2C11_A647_CD133_Antibody_FC_1_080715
Mouse CD133 transfected cells were stained with CD133 (clone 315-2C11) Alexa Fluor® 647 (filled histogram) or rat IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 315-2C11_A647_CD133_Antibody_FC_1_080715
    Mouse CD133 transfected cells were stained with CD133 (clone 315-2C11) Alexa Fluor® 647 (filled histogram) or rat IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 315-2C11_A647_CD133_Antibody_FC_2_080715
  • 315-2C11_A647_CD133_Antibody_FC_3_080715
Compare all formats See Alexa Fluor® 647 spectral data
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141215 25 µg $159
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141216 100 µg $370
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Description

CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed
Product Citations
  1. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  2. Fernandez HR, et al. 2018. Cell Death Differ. 25:1239. PubMed
RRID
AB_2566012 (BioLegend Cat. No. 141215)
AB_2566012 (BioLegend Cat. No. 141216)

Antigen Details

Structure
120 kD pentaspan transmembrane glycoprotein
Distribution
Hematopoietic stem cells, epithelial progenitor cells, leukemia cells, brain tumor cells, kidney, pancreas, placenta, and fetal liver cells
Function
Biological function unknown
Cell Type
Epithelial cells, Hematopoietic stem and progenitors, Leukemia, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Singh SK, et al. 2004. Nature 18:396.
2. Falini B, et al. 2005. New Engl. J. Med. 352:254.
3. Bonanno G, et al. 2004. Transfusion 44:1087.
4. Gaipa G, et al. 2002. Haematologica 87:347.
5. Wuchter C, et al. 2001. Haematologica 86:154.

Gene ID
19126 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/17/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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