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Purified anti-human/mouse CD49f Antibody

Pricing & Availability
Clone
GoH3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Isotype
Rat IgG2a, κ
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Product Citations
publications
GoH3_Purified_071307
Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC
  • GoH3_Purified_071307
    Human peripheral blood lymphocytes stained with purified GoH3, followed by anti-rat IgGs FITC
  • zGoH3_IF_073012
    MDA-MB-231 breast cancer cells were stained with anti-CD49f (clone GoH3) followed by DyLight™ 649 Goat anti-rat Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
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313602 100 µg $119
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Description

CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Cat, Cow, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F, IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Tremblay M, et al. 2020. Elife. 9: . PubMed
  3. Sekihara K, et al. 2022. Cancer Cell Int. 22:391. PubMed
  4. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  5. García–Mariscal A, et al. 2018. Oncogene. 37:847. PubMed
  6. Shannon JL, et al. 2022. Bio Protoc. 12:. PubMed
  7. Lowndes M, et al. 2016. Stem Cell Reports. 7:126-137. PubMed
  8. Leelatian N, et al. 2020. eLife. 9:e56879.. PubMed
  9. Heinrich A, et al. 2021. Cell Rep. 37:109885. PubMed
  10. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  11. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  12. Sivasubramaniyan K, et al. 2015. Glycobiology. 25: 902-917. PubMed
  13. Engelbrecht E, et al. 2020. eLife. 9:e52690.. PubMed
  14. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  15. Liu LP et al. 2019. Cell Rep. 27(2):455-466 . PubMed
  16. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  17. Romagnoli M, et al. 2020. Development. 147:. PubMed
  18. Seino T et al. 2018. Cell stem cell. 22(3):454-467 . PubMed
  19. Xi L, et al. 2020. Elife. 9:00. PubMed
  20. Mok KW et al. 2018. Developmental cell. 48(1):32-48 . PubMed
  21. Nanki K, et al. 2018. Cell. 174:856. PubMed
  22. Ishibashi R, et al. 2022. Sci Rep. 12:17775. PubMed
  23. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  24. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  25. Kawasaki K, et al. 2020. Cell. 183(5):1420-1435.e21. PubMed
  26. Barbar L, et al. 2020. Neuron. 107(3):436-453.e12. PubMed
  27. Doffe F, et al. 2022. Cancers (Basel). 14:. PubMed
  28. Lai K, et al. 2012. Mol Endocrinol. 26:52:00. PubMed
  29. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
RRID
AB_345296 (BioLegend Cat. No. 313602)

Antigen Details

Structure
Integrin family, associates with β1 or β4, 120 kD
Distribution

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Function
Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand/Receptor
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
UniProt
View information about CD49f on UniProt.org

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For Research Use Only. Not for diagnostic or therapeutic use.

 

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