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Alexa Fluor® 647 anti-mouse CD41 Antibody

Pricing & Availability
Clone
MWReg30 (See other available formats)
Regulatory Status
RUO
Other Names
Fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb, CD41a
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MWReg30_A647_CD41_Antibody_091718
BALB/c mouse platelets were stained with CD41 (clone MWReg30) Alexa Fluor® 647 (filled histogram) or rat IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • MWReg30_A647_CD41_Antibody_091718
    BALB/c mouse platelets were stained with CD41 (clone MWReg30) Alexa Fluor® 647 (filled histogram) or rat IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
133933 25 µg $112
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133934 100 µg $288
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Description

CD41, also known as integrin α2b and GPIIb, is a transmembrane glycoprotein that is expressed by platelets and megakaryocytes. It was reported that CD41 is also expressed on hematopoietic progenitors. CD41 associates with CD61 (integrin β3) to form complexes that interact with fibrinogen, fibronectin, von Willebrand factor, and thrombin. CD41 is required for platelet adhesion and aggregation. Defect of CD41 leads to disorders of coagulation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: depletion of platelets and functional assay in vivo.4,7 The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies (Cat. No. 133939).

Application References

(PubMed link indicates BioLegend citation)
  1. Nieswandt B, et al. 1999. Blood 94:684.
  2. Teeling JL, et al. 2001. Blood 98:1095.
  3. Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
  4. Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
  5. Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
  6. van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
  7. Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)
Product Citations
  1. He H, et al. 2020. Sci Adv. 6:00. PubMed
  2. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  3. Nicolai L, et al. 2021. Haematologica. Online ahead of print. PubMed
RRID
AB_2750527 (BioLegend Cat. No. 133933)
AB_2750527 (BioLegend Cat. No. 133934)

Antigen Details

Structure
A transmembrane glycoprotein that is expressed by platelets and megakaryocytes
Distribution

Platelets, megakaryocytes, and hematopoietic progenitors

Function
Associate with CD61 (integrin β3) to form a complex which plays a major role in platelet adhesion and aggregation
Ligand/Receptor
Fibrinogen, fibronectin, von Willebrand factor, thrombin
Cell Type
Hematopoietic stem and progenitors, Megakaryocytes, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bakewell SJ, et al. 2003. P. Natl. Acad. Sci. USA 100:14205.
2. Phillips DR, et al. 1991. Cell. 65:359.

Gene ID
16399 View all products for this Gene ID
UniProt
View information about CD41 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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