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Alexa Fluor® 647 anti-mouse CD49e Antibody

Pricing & Availability
Clone
5H10-27(MFR5) (See other available formats)
Regulatory Status
RUO
Other Names
α5 integrin, VLA-5 α chain, Integrin α5, Fnra, Itga5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
5H10-27_Alx647_100507
Balb/c mouse bone marrow stained with 5H10-27 Alexa Fluor® 647
  • 5H10-27_Alx647_100507
    Balb/c mouse bone marrow stained with 5H10-27 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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103811 25 µg $101
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103812 100 µg $229
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Description

CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
(C57BL/6 x A/J)F1 mouse mast cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818).

Application References

(PubMed link indicates BioLegend citation)
  1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
  2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
  3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
  4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
  5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
  6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
  7. Cuklerman E, et al. 2001. Science 294:1708. (IHC)
  8. Jia W, et al. 2005. Blood 106:3854. (FC)
RRID
AB_528840 (BioLegend Cat. No. 103811)
AB_528840 (BioLegend Cat. No. 103812)

Antigen Details

Structure
Integrin family, associates with integrin β1, 135 kD
Distribution

Thymocytes, activated T cells, mast cells

Function
Adhesion, costimulation
Ligand/Receptor
Fibronectin
Cell Type
Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.

Gene ID
16402 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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