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Alexa Fluor® 647 anti-Tau Phospho (Ser396) Antibody

Pricing & Availability
Clone
PHF-13 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
PHF-13_A647_tau-phospho-ser396_Antibody_1_121819.png
IHC staining of Alexa Fluor® 647 anti-Tau Phospho (Ser396) (clone PHF-13) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • PHF-13_A647_tau-phospho-ser396_Antibody_1_121819.png
    IHC staining of Alexa Fluor® 647 anti-Tau Phospho (Ser396) (clone PHF-13) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
829005 25 µg $188
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829006 100 µg $405
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Description

Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated tau protein. Predominantly expressed in axons, alternatively spliced forms of tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-tau differs from normal tau by its abnormal hyperphosphorylation, which results in decreased binding of tau to microtubules. The decreased affinity of PHF-tau for microtubules, coupled with reduced levels of normal tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of tau is believed to be a key event in the pathogenesis of Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The PHF-13 antibody was raised using PHF-tau preparations from human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody is effective in formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P) and immunoblotting (WB).

The PHF-13 antibody reacts with human tau phosphorylated at Ser 396. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lasagna-Reeves CA, et al. 2012. FASEB J. (26):1946 (WB) (PUBMED)
  2. Lauretti E, et al. 2017. Ann Clin Transl Neurol. 4(8): 564 (WB, IHC)
  3. Li JG, et al. 2017. Sci Rep. (7):46002 (WB, IHC)
  4. Li JG, et al. 2014. Ann Neurol. 75(6):851 (WB, IHC)
RRID
AB_2820133 (BioLegend Cat. No. 829005)
AB_2820133 (BioLegend Cat. No. 829006)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, Tau
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho Ser396 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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