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Purified anti-Tau Phospho (Ser396) Antibody (Previously Covance catalog# MMS-546P)

Pricing & Availability
Clone
PHF-13 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Previously
Covance Catalog# MMS-546P
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
PHF-13_Pure_tau-phospho-ser396_Antibody_1_062218
IHC staining of purified anti-Tau Phospho (Ser396) antibody (clone PHF-13) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • PHF-13_Pure_tau-phospho-ser396_Antibody_1_062218
    IHC staining of purified anti-Tau Phospho (Ser396) antibody (clone PHF-13) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • PHF-13_Pure_tau-phospho-ser396_Antibody_2_062218
    IHC staining of purified anti-Tau Phospho (Ser396) antibody (clone PHF-13) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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829003 25 µL $141
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829001 100 µL $357
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Description

Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated tau protein. Predominantly expressed in axons, alternatively spliced forms of tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-tau differs from normal tau by its abnormal hyperphosphorylation, which results in decreased binding of tau to microtubules. The decreased affinity of PHF-tau for microtubules, coupled with reduced levels of normal tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of tau is believed to be a key event in the pathogenesis of Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The PHF-13 antibody was raised using PHF-tau preparations from human brain.
Formulation
Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The PHF-13 antibody reacts with human tau phosphorylated at Ser 396. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lasagna-Reeves CA, et al. 2012. FASEB J. (26):1946 (WB) PubMed
  2. Lauretti E, et al. 2017. Ann Clin Transl Neurol. 4(8): 564 (WB, IHC-P)
  3. Li JG, et al. 2017. Sci Rep. (7):46002 (WB, IHC-P)
  4. Li JG, et al. 2014. Ann Neurol. 75(6):851 (WB, IHC-P)
Product Citations
  1. Campbell NB, et al. 2023. Int J Mol Sci. 24:. PubMed
RRID
AB_2564925 (BioLegend Cat. No. 829003)
AB_2564925 (BioLegend Cat. No. 829001)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, Tau
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho Ser396 on UniProt.org
Go To Top Version: 6    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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