Alexa Fluor® 660 anti-human CD8a Antibody, CD8, SK1

Pricing & Availability

Clone: SK1 (See other available formats)
Regulatory Status: RUO
Other Names: T8, Leu2
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK1_AlexaFlour660_CD8a_Antibody_050423 — Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD8a (clone SK1) Alexa Flour® 660 (left) or stained with anti-human CD3 FITC only (right).

Compare all formats See Alexa Fluor® 660 spectral data

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344779

25 tests

$170

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344780

100 tests

$390

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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 660 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 660 has an excitation maximum of 663 nm, and a maximum emission of 690 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics⁸, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
RRID: AB_2941525 (BioLegend Cat. No. 344779)
AB_2941525 (BioLegend Cat. No. 344780)

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 925 View all products for this Gene ID
UniProt: View information about CD8 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD8 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 647 anti-human CD8	SK1	FC
Brilliant Violet 650™ anti-human CD8	SK1	FC
Purified anti-human CD8	SK1	FC,IHC-F
FITC anti-human CD8	SK1	FC
PE anti-human CD8	SK1	FC
PerCP anti-human CD8	SK1	FC
PerCP/Cyanine5.5 anti-human CD8	SK1	FC
PE/Cyanine7 anti-human CD8	SK1	FC
APC/Cyanine7 anti-human CD8	SK1	FC
Alexa Fluor® 488 anti-human CD8	SK1	FC,SB
Pacific Blue™ anti-human CD8	SK1	FC
Biotin anti-human CD8	SK1	FC
APC anti-human CD8	SK1	FC
Alexa Fluor® 700 anti-human CD8	SK1	FC
Purified anti-human CD8 (Maxpar® Ready)	SK1	FC,CyTOF®
Brilliant Violet 510™ anti-human CD8	SK1	FC
Brilliant Violet 711™ anti-human CD8	SK1	FC
Brilliant Violet 785™ anti-human CD8	SK1	FC
Brilliant Violet 605™ anti-human CD8	SK1	FC
PE/Dazzle™ 594 anti-human CD8	SK1	FC
PE anti-human CD8	SK1	FC
APC/Fire™ 750 anti-human CD8	SK1	FC
APC anti-human CD8	SK1	FC
Brilliant Violet 421™ anti-human CD8	SK1	FC
Pacific Blue™ anti-human CD8	SK1	FC
TotalSeq™-A0046 anti-human CD8	SK1	PG
TotalSeq™-C0046 anti-human CD8	SK1	PG
Brilliant Violet 750™ anti-human CD8	SK1	FC
TotalSeq™-B0046 anti-human CD8	SK1	PG
Spark Blue™ 550 anti-human CD8	SK1	FC
PE/Cyanine7 anti-human CD8	SK1	FC
FITC anti-human CD8	SK1	FC
APC/Fire™ 810 anti-human CD8	SK1	FC
PE/Fire™ 640 anti-human CD8	SK1	FC
PE/Fire™ 700 anti-human CD8	SK1	FC
PerCP anti-human CD8	SK1	FC
PerCP/Cyanine5.5 anti-human CD8	SK1	FC
TotalSeq™-D0046 anti-human CD8	SK1	PG
APC/Fire™ 750 anti-human CD8	SK1	FC
GMP APC anti-human CD8	SK1	FC
PE/Cyanine5 anti-human CD8 Antibody	SK1	FC
Spark UV™ 387 anti-human CD8	SK1	FC
GMP PE anti-human CD8	SK1	FC
GMP PE/Cyanine7 anti-human CD8	SK1	FC
Spark NIR™ 685 anti-human CD8	SK1	FC
KIRAVIA Blue 520™ anti-human CD8	SK1	FC
GMP FITC anti-human CD8	SK1	FC
GMP Pacific Blue™ anti-human CD8	SK1	FC
GMP PerCP anti-human CD8	SK1	FC
Spark Violet™ 500 anti-human CD8	SK1	FC
GMP APC/Fire™ 750 anti-human CD8	SK1	FC
GMP PerCP/Cyanine 5.5 anti-human CD8	SK1	FC
Alexa Fluor® 660 anti-human CD8a	SK1	FC
Spark Blue™ 515 anti-human CD8	SK1	FC
Spark Blue™ 574 anti-human CD8	SK1	FC
Spark Violet™ 538 anti-human CD8	SK1	FC
PE/Fire™ 810 anti-human CD8	SK1	FC
Spark YG™ 593 anti-human CD8	SK1	FC
PerCP/Fire™ 806 anti-human CD8	SK1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/20/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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