- Clone
- SK1 (See other available formats)
- Other Names
- T8, Leu2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
260176 | 100 tests | $429 |
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions.
- Concentration
- 200 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
-
Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Disclaimer
-
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
-
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD8 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Brilliant Violet 650™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Purified anti-human CD8
Human peripheral blood lymphocytes stained with SK1, followe... -
FITC anti-human CD8
Human peripheral blood lymphocytes stained with SK1 FITC -
PE anti-human CD8
Human peripheral blood lymphocytes stained with SK1 PE. -
PerCP anti-human CD8
Human peripheral blood lymphocytes stained with SK1 PerCP -
PerCP/Cyanine5.5 anti-human CD8
Human peripheral blood lymphocytes stained with CD8 (clone S... -
PE/Cyanine7 anti-human CD8
Human peripheral blood lymphocytes stained with SK1 PE/Cyani... -
APC/Cyanine7 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Alexa Fluor® 488 anti-human CD8
Human peripheral blood lymphocytes stained with SK1 Alexa Fl... Confocal image of human lymph node sample acquired using the... Confocal image of human metastatic lymph node sample acquire... -
Pacific Blue™ anti-human CD8
Human peripheral blood lymphocytes stained with SK1 Pacific ... -
Biotin anti-human CD8
Human peripheral blood lymphocytes stained with SK1 biotin, ... -
APC anti-human CD8
Human peripheral blood lymphocytes stained with SK1 APC -
Alexa Fluor® 700 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Purified anti-human CD8 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 151Eu-an... -
Brilliant Violet 510™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Brilliant Violet 711™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Brilliant Violet 785™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Brilliant Violet 605™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE/Dazzle™ 594 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD8
Human peripheral blood lymphocytes were stained with CD3 PE ... -
APC anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 421™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
Pacific Blue™ anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0046 anti-human CD8
-
TotalSeq™-C0046 anti-human CD8
-
Brilliant Violet 750™ anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
TotalSeq™-B0046 anti-human CD8
-
Spark Blue™ 550 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE/Cyanine7 anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
FITC anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 810 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE/Fire™ 640 anti-human CD8
Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE/Fire™ 700 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-D0046 anti-human CD8
-
APC/Fire™ 750 anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine5 anti-human CD8 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark UV™ 387 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
Spark NIR™ 685 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
KIRAVIA Blue 520™ anti-human CD8
Human peripheral blood lymphocytes stained with anti-human C... -
GMP FITC anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
GMP Pacific Blue™ anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
GMP PerCP anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 500 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP APC/Fire™ 750 anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
GMP PerCP/Cyanine 5.5 anti-human CD8
Typical results from human peripheral blood lymphocytes stai... -
Alexa Fluor® 660 anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 515 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 574 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Violet™ 538 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark YG™ 593 anti-human CD8
Human peripheral blood lymphocytes were stained with anti-hu...
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