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Anti-GPP130 Antibody (Previously Covance catalog# PRB-144C)

Pricing & Availability
Clone
Poly19238 (See other available formats)
Regulatory Status
RUO
Other Names
GIMPC, GOLPH4, Golgi Integral Membrane Protein 4, Golgi Phosphoprotein 4, Type Ii Golgi Membrane Protein, Golgi Phosphoprotein Of 130 kD, Golgi Integral Membrane Protein, Cis, 130 kD Golgi-Localized Phosphoprotein, Cis Golgi-
Previously
Covance Catalog# PRB-144C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly19238_Anti_GPP130_Antibody_012318
Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B-D) GPP130 primary Antibody (Poly19238). Alexa Fluor® 488 (Green) Donkey anti-Rabbit IgG (Cat. No. 406416) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/20, and (B-D) is 1/60. Concentrations for (A, B) is 1/100, (C) is 1/250, (D) is 1/500.
  • Poly19238_Anti_GPP130_Antibody_012318
    Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B-D) GPP130 primary Antibody (Poly19238). Alexa Fluor® 488 (Green) Donkey anti-Rabbit IgG (Cat. No. 406416) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/20, and (B-D) is 1/60. Concentrations for (A, B) is 1/100, (C) is 1/250, (D) is 1/500.
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923801 200 µL $399
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Description

The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain. GPP130 has been a revealing marker of early Golgi vesicle trafficking.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Non-Human Primates, Rabbit, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against a recombinant GST-GPP130 fusion protein.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:100 - 1:250 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunoprecipitation (IP).

*Predicted MW = 130 kD


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Puri S, et al. 2002. Traffic. 3:641.
  2. Bachert C, et al. 2001. Mol Biol Cell. 12:3152.
  3. Linstedt AD, et al. 1997. Mol Biol Cell. 8:1073.
Product Citations
  1. Bui S, et al. 2022. Methods Mol Biol. 2557:453. PubMed
  2. Rebane AA, et al. 2020. FEBS Lett. 594:1132. PubMed
  3. Yue X, et al. 2021. Commun Biol. 4:1370. PubMed
  4. Lee G, et al. 2016. J Cell Biol. 215(5):705-718. PubMed
  5. Wakana Y, et al. 2021. J Cell Biol. 220: . PubMed
  6. Hasegawa H, et al. 2016. Biochim Biophys Acta. 1863: 1534-1551. PubMed
  7. Choi EK, et al. 2019. FASEB J. 33:2228. PubMed
  8. Groux-Degroote S, et al. 2018. FEBS J. 285:3442. PubMed
  9. Choi EK, et al. 2018. Sci Rep. 8:3163. PubMed
RRID
AB_2565442 (BioLegend Cat. No. 923801)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers, Phospho-Proteins
Gene ID
27333 View all products for this Gene ID
UniProt
View information about GPP130 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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